Rapid cytopathic effects of Clostridium perfringens beta-toxin on porcine endothelial cells

Gurtner, C; Popescu, F; Wyder, M; Sutter, E; Zeeh, F; Frey, J; von Schubert C., ; Posthaus, H (2010). Rapid cytopathic effects of Clostridium perfringens beta-toxin on porcine endothelial cells. Infection and immunity, 78(7), pp. 2966-73. New York, N.Y.: American Society for Microbiology 10.1128/IAI.01284-09

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Clostridium perfringens type C isolates cause fatal, segmental necro-hemorrhagic enteritis in animals and humans. Typically, acute intestinal lesions result from extensive mucosal necrosis and hemorrhage in the proximal jejunum. These lesions are frequently accompanied by microvascular thrombosis in affected intestinal segments. In previous studies we demonstrated that there is endothelial localization of C. perfringens type C beta-toxin (CPB) in acute lesions of necrotizing enteritis. This led us to hypothesize that CPB contributes to vascular necrosis by directly damaging endothelial cells. By performing additional immunohistochemical studies using spontaneously diseased piglets, we confirmed that CPB binds to the endothelial lining of vessels showing early signs of thrombosis. To investigate whether CPB can disrupt the endothelium, we exposed primary porcine aortic endothelial cells to C. perfringens type C culture supernatants and recombinant CPB. Both treatments rapidly induced disruption of the actin cytoskeleton, cell border retraction, and cell shrinkage, leading to destruction of the endothelial monolayer in vitro. These effects were followed by cell death. Cytopathic and cytotoxic effects were inhibited by neutralization of CPB. Taken together, our results suggest that CPB-induced disruption of endothelial cells may contribute to the pathogenesis of C. perfringens type C enteritis.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Frey, Joachim

ISSN:

0019-9567

Publisher:

American Society for Microbiology

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:32

Last Modified:

17 Mar 2015 21:19

Publisher DOI:

10.1128/IAI.01284-09

Web of Science ID:

000278830200008

URI:

https://boris.unibe.ch/id/eprint/12341 (FactScience: 218667)

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