Smole, I; Thomann, A; Frey, J; Perreten, V (2010). Repression of common bull sperm flora and in vitro impairment of sperm motility with Pseudomonas aeruginosa introduced by contaminated lubricant. Reproduction in domestic animals, 45(4), pp. 737-42. Berlin: Blackwell 10.1111/j.1439-0531.2008.01319.x
Full text not available from this repository.Semen collected from clinically healthy bulls at an artificial insemination centre was examined for bacterial diversity. While bacteria that are normally present in the common flora of bovine semen were absent, such as Mycoplasma sp., Proteus sp. and Corynebacterium sp., all semen samples contained an unusually high number of Pseudomonas aeruginosa strains. Analysis via pulsed field gel electrophoresis demonstrated that one particular P. aeruginosa strain, present in a sealed bottle of lubricant, was widespread in bull semen. This strain was shown to secrete substances that inhibited both the growth of bacteria constituting the normal bull sperm flora and the motility of spermatozoa in vitro. This study demonstrated that commercially available lubricants might contain bacteria that can spread amongst breeding bulls and affect the quality of semen. Bacteriological controls and species' identification are necessary at several production levels, including lubricants and extenders, to ensure high semen quality and avoid the spread of pathogens.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology |
UniBE Contributor: |
Frey, Joachim, Perreten, Vincent |
ISSN: |
0936-6768 |
Publisher: |
Blackwell |
Language: |
English |
Submitter: |
Factscience Import |
Date Deposited: |
04 Oct 2013 14:32 |
Last Modified: |
05 Dec 2022 14:10 |
Publisher DOI: |
10.1111/j.1439-0531.2008.01319.x |
Web of Science ID: |
000279841200028 |
URI: |
https://boris.unibe.ch/id/eprint/12345 (FactScience: 218671) |
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