Use of an indirect enzyme-linked immunosorbent assay for detection of antibodies in sheep naturally infected with Salmonella Abortusovis

Wirz-Dittus, S; Belloy, L; Doherr, M G; Hussy, D; Sting, R; Gabioud, P; Waldvogel, A S (2010). Use of an indirect enzyme-linked immunosorbent assay for detection of antibodies in sheep naturally infected with Salmonella Abortusovis. Journal of veterinary diagnostic investigation, 22(4), pp. 531-6. Columbia, Mo.: American Association of Veterinary Laboratory Diagnosticians AAVLD 10.1177/104063871002200406

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An indirect enzyme-linked immunosorbent assay (ELISA) was modified and validated to detect antibodies against Salmonella Abortusovis in naturally infected sheep. The ELISA was validated with 44 positive and 45 negative control serum samples. Compared with the immunoblot, the sensitivity and specificity of the assay were 98% and 100%, respectively. To follow antibody levels over time, samples from 12 infected ewes were collected at 1, 3, and 10 months after abortion. All animals showed antibody levels above the cutoff value throughout the observation period. One and 3 months after abortion, high antibody levels could be detected in all but one animal, whereas after 10 months, 9 animals had markedly lower but still positive antibody levels. The test characteristics and evidence for the persistence of detectable antibody levels in all infected animals for up to 10 months indicates that the ELISA can be used for herd surveillance testing.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Doherr, Marcus and Hüssy, Daniela

ISSN:

1040-6387

Publisher:

American Association of Veterinary Laboratory Diagnosticians AAVLD

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:32

Last Modified:

17 Mar 2015 21:19

Publisher DOI:

10.1177/104063871002200406

Web of Science ID:

000279970300006

URI:

https://boris.unibe.ch/id/eprint/12361 (FactScience: 218689)

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