Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays

Lauber, Beatrice; Marti, Eliane Isabelle; Plattet, Philippe; Urwyler, A.; Rai, M.F.; Schmidt, M.F.; Welle, Monika Maria; Zurbriggen, Andreas; Janda, Jozef (2010). Distinct reactivities of interleukin-4-specific antibodies with recombinant and native canine interleukin-4 in various assays. Veterinary immunology and immunopathology, 137(3-4), pp. 310-6. Amsterdam: Elsevier 10.1016/j.vetimm.2010.05.015

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Interleukin 4 (IL-4) plays a central role in immune responses to parasites and allergens. IL-4 drives the differentiation of naive T cells into Th2 cells and regulates immunoglobulin class switching to IgE.Little is known about the role of IL-4 in canine allergies and parasite infections. Most of the information derives from measurement of IL-4 mRNA expression in dog tissues, but detection of IL-4 protein has been difficult so far, probably due to low sensitivity of available methods. Antibodies (Ab) specific for canine IL-4 are available from various sources, but these Ab have been produced against recombinant Escherichia coli-expressed canine IL-4 and there is only limited information on their reactivities with native canine IL-4. Therefore, in the present study, we tested six available canine IL-4-specific Ab for their reactivities with recombinant canine IL-4 expressed in E. coli (rec.IL-4) or in mammalian cells (mam.IL-4), and with supernatants from stimulated canine peripheral blood mononuclear cells (PBMCs) using several detection methods, including Western blotting, ELISA, cytokine bead assay, and intracellular IL-4 staining. Additionally, we tested a bovine IL-4-specific antibody that has been previously shown to cross-react with canine IL-4. All tested Ab except anti-bovine IL-4 reacted with rec.IL-4, and most of them reacted with mam.IL-4. However, only the cytokine bead assay was sensitive enough to allow the detection of IL-4 in supernatants of canine PBMCs.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Lauber, Beatrice; Marti, Eliane Isabelle; Plattet, Philippe; Welle, Monika Maria; Zurbriggen, Andreas and Janda, Jozef

Subjects:

600 Technology > 630 Agriculture

ISSN:

0165-2427

Publisher:

Elsevier

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:32

Last Modified:

16 Feb 2015 15:45

Publisher DOI:

10.1016/j.vetimm.2010.05.015

Web of Science ID:

000282402700017

URI:

https://boris.unibe.ch/id/eprint/12377 (FactScience: 218710)

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