Superior bone-inducing potential of rhBMP9 compared to rhBMP2.

Kobayashi, Masako; Abd El Raouf, Mustafa; Saulacic, Nikola; Kobayashi, Eizaburo; Zhang, Yufeng; Schaller, Benoît; Miron, Richard John (2018). Superior bone-inducing potential of rhBMP9 compared to rhBMP2. Journal of biomedical materials research. Part A, 106(6), pp. 1561-1574. John Wiley & Sons 10.1002/jbm.a.36359

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Recombinant human bone morphogenic protein (rhBMP) 9 has recently been reported to have more osteopromotive potential in vitro when compared to rhBMP2. The aim of the present study was to investigate the bone-inducing potential of rhBMP2 and rhBMP9. We compared rhBMP2, rhBMP7, and rhBMP9 at five different concentrations and showed convincingly that rhBMP9 possesses much greater potential for osteoblast differentiation even at 20 times lower concentrations in vitro. We further show that Noggin, an inhibitor for rhBMP2-induced osteogenesis, did not alter rhBMP9-induced osteogenesis. Thereafter, we show for the first time that rhBMP9 loaded onto atelo-collagen membranes is osteoinductive and has greater potential to form ectopic bone formation when compared to rhBMP2 even at four times lower doses. Similarly new bone formation of rhBMP2 and 9 when loaded on deproteinized bovine bone mineral (DBBM) was investigated in a rabbit calvarial defect. At 8 weeks, both rhBMP2 and rhBMP9 induced significantly higher new bone formation when compared to DBBM alone samples. Interestingly, once again four times lower dose of rhBMP9 group induced comparable or even greater levels of new bone height and new bone area when compared to the rhBMP2 group. The present study revealed that (1) rhBMP9 is capable of inducing ectopic new bone formation in vivo and (2) up to four times lower doses of rhBMP9 may be utilized to regenerate same-size bone defects when compared to rhBMP2. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1561-1574, 2018.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

UniBE Contributor:

Kobayashi, Masako; Saulacic, Nikola; Kobayashi, Eizaburo; Schaller, Benoît and Miron, Richard John

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1549-3296

Publisher:

John Wiley & Sons

Language:

English

Submitter:

Caroline Dominique Zürcher

Date Deposited:

21 Feb 2019 10:21

Last Modified:

04 Dec 2019 02:02

Publisher DOI:

10.1002/jbm.a.36359

Related URLs:

PubMed ID:

29396910

Uncontrolled Keywords:

BMP2 BMP9 Bone induction Bone morphogenetic proteins DBBM atelo-collagen

BORIS DOI:

10.7892/boris.123936

URI:

https://boris.unibe.ch/id/eprint/123936

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