Micronucleus formation in human cancer cells is biased by chromosome size.

Bochtler, Tilmann; Kartal-Kaess, Mutlu; Granzow, Martin; Hielscher, Thomas; Cosenza, Marco R; Herold-Mende, Christel; Jauch, Anna; Krämer, Alwin (2019). Micronucleus formation in human cancer cells is biased by chromosome size. Genes, chromosomes & cancer, 58(6), pp. 392-395. Wiley 10.1002/gcc.22707

[img] Text
Bochtler_et_al-2019-Genes,_Chromosomes_and_Cancer.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Chromosomal instability is one of the hallmarks of cancer and caused by chromosome missegregation during mitosis, a process frequently associated with micronucleus formation. Micronuclei are formed when chromosomes fail to join a daughter nucleus during cell division and are surrounded by their own nuclear membrane. Although it has been commonly assumed that the gain or loss of specific chromosomes is random during compromised cell division, recent data suggest that the size of chromosomes can impact on chromosome segregation fidelity. To test whether chromosome missegregation rates scale with chromosome size in primary human cancer cells, we assessed chromosome sequestration into micronuclei in patient-derived primary NCH149 glioblastoma cells, which display high-level numerical chromosome instability (CIN), pronounced spontaneous micronucleus formation but virtually no structural CIN. The cells were analysed by interphase fluorescence-in-situ-hybridization (FISH) using chromosome-specific painting probes for all chromosomes. Overall, 33% of early passage NCH149 cells harbored micronuclei. Entrapment within a micronucleus clearly correlated with chromosome size with larger chromosomes being significantly more frequently missegregated into micronuclei than smaller chromosomes in primary glioblastoma cells. These findings extend the concept that chromosome size determines segregation fidelity by implying that size-specific micronucleus entrapment occurs in primary human cancer cells as well. This article is protected by copyright. All rights reserved.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Department of Gynaecology, Paediatrics and Endocrinology (DFKE) > Clinic of Paediatric Medicine

UniBE Contributor:

Kartal-Kaess, Mutlu


600 Technology > 610 Medicine & health








Karen Lidzba

Date Deposited:

26 Feb 2019 08:51

Last Modified:

25 Oct 2019 06:11

Publisher DOI:


PubMed ID:


Additional Information:

Bochtler & Kartal-Kaess are joint first authors

Uncontrolled Keywords:

Micronucleus cancer cells chromosome size





Actions (login required)

Edit item Edit item
Provide Feedback