Comparative expression patterns of selected membrane transporters in differentiated BeWo and human primary trophoblast cells.

Kallol, Sampada Arvindrao; Moser-Haessig, Ruedi; Ontsouka, Edgar; Albrecht, Christiane (2018). Comparative expression patterns of selected membrane transporters in differentiated BeWo and human primary trophoblast cells. Placenta, 72-73, pp. 48-52. Elsevier 10.1016/j.placenta.2018.10.008

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Although placental membrane transporters have an important impact on materno-fetal nutrient transfer, placental cell models are poorly characterized regarding transporter expression. We assessed the mRNA expression of 26 physiologically important solute carriers and ABC transporters in BeWo (b30 clone) and primary human trophoblast cells (PHT) before and after syncytialization. 77% of the transporters showed similar mRNA expression changes between BeWo and PHT after syncytialization. Selected transporters, however, were either lacking in BeWo or showed different trends after syncytialization. In conclusion, BeWo cells generally represent an apt model for transporter studies, but their suitability should be confirmed for each transporter by comparison with PHT.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Other Institutions > NCCR TransCure
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Kallol, Sampada Arvindrao; Ontsouka, Edgar and Albrecht, Christiane

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

0143-4004

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

27 Feb 2019 11:12

Last Modified:

27 Feb 2019 11:12

Publisher DOI:

10.1016/j.placenta.2018.10.008

PubMed ID:

30501881

Uncontrolled Keywords:

ABC transporter BeWo b30 clone Cell fusion Forskolin Placenta SLC transporter Syncytialization Trophoblast

BORIS DOI:

10.7892/boris.124478

URI:

https://boris.unibe.ch/id/eprint/124478

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