Development of a whole plant bioassay to test effects of potentized calcium carbonate in pillule formulation.

Lutz, Bianka; Heer, Iris; Katzensteiner, Ramona; Raak, Christa; Wolf, Ursula; Heusser, Peter; Frass, Michael; Baumgartner, Stephan (2018). Development of a whole plant bioassay to test effects of potentized calcium carbonate in pillule formulation. Complementary therapies in medicine, 40, pp. 13-21. Elsevier 10.1016/j.ctim.2018.06.005

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OBJECTIVES From a pharmaceutical point of view, we see a need to develop stable preclinical test systems to identify and investigate effects of potentized remedies as used in Anthroposophic Medicine and Homeopathy. We evaluated a plant bioassay regarding its capacity to distinguish homeopathic remedies from placebo, applied as sucrose pillules. METHODS Pea seed (Pisum sativum L) was soaked for 24 hours in water with dissolved homeopathic or placebo pillules, or in water only. Shoot length was measured 14 days after planting and treatment groups were compared by analysis of variance (ANOVA). The stability of the system was validated by systematic negative control experiments. RESULTS The system is suitable to test a common application form - sucrose pillules - of a potentized preparation without influence of the pharmaceutical carrier substance. A screening of 13 potentized preparations revealed Calcium carbonicum to affect pea shoot growth (p < 0.05). Three independent series of main experiments were performed with potentized Calcium carbonicum to assess reproducibility. Meta-analysis of all data revealed significant effects of Calcium carbonicum 12c and 30c on pea shoot growth (p < 0.05), which were however dependent on the date of experiment and/or the experimental series. CONCLUSIONS Potentized Calcium carbonicum, applied as sucrose pillules, influenced pea shoot growth in the assay investigated. However, due to the small effect size and due to the modulation of the effects by still unknown external factors, further optimization of this bioassay is necessary to be used in pharmaceutical quality control or in investigating the biological or pharmaceutical mode of action of potentized preparations.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Medical Education > Institute of Complementary and Integrative Medicine (IKIM)

UniBE Contributor:

Wolf, Ursula; Heusser, Peter and Baumgartner, Stephan

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0965-2299

Publisher:

Elsevier

Language:

English

Submitter:

Yasmine Bühlmann

Date Deposited:

17 Apr 2019 14:08

Last Modified:

24 Oct 2019 04:55

Publisher DOI:

10.1016/j.ctim.2018.06.005

PubMed ID:

30219438

Uncontrolled Keywords:

Anthroposophic Medicine Homeopathy calcium carbonicum dwarf peas plant bioassay reproducibility

BORIS DOI:

10.7892/boris.125004

URI:

https://boris.unibe.ch/id/eprint/125004

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