Bone-Conditioned Medium Obtained From Calvaria, Mandible, and Tibia Cause an Equivalent TGF-β1 Response In Vitro.

Hatami Kia, Babak; Mendes, João Rui Galvão; Müller, Heinz-Dieter; Heimel, Patrick; Gruber, Reinhard (2018). Bone-Conditioned Medium Obtained From Calvaria, Mandible, and Tibia Cause an Equivalent TGF-β1 Response In Vitro. Journal of craniofacial surgery, 29(3), pp. 553-557. Lippincott Williams & Wilkins 10.1097/SCS.0000000000004251

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Bones with different embryological origin and mode of ossification are supposed to vary in their capacity for supporting graft consolidation. The aim of the current pilot study was to assess the TGF-β1 activity of bone chips obtained from distinct anatomic locations. Conditioned medium was prepared from bone chips harvested from pig calvaria, mandible, and tibia. Human oral fibroblasts were exposed to bone-conditioned medium (BCM) followed by reverse transcriptase polymerase chain reaction of the TGF-β1 target genes. Also an immunoassay for interleukin 11 (IL-11) and TGF-β1 was performed. The impact of BCM on alkaline phosphatase activity was determined with murine MC3T3-E1 osteogenic cells. The authors report here that BCM contains TGF-β1 in the ng/mL range. Bone chips prepared from pig calvaria, mandible, and tibia femur had a similar capacity for increasing the expression of the TGF-β1 target genes IL-11, NOX4, and PRG4. Correspondingly, immunoassays revealed similar production of IL-11 by human oral fibroblasts. Furthermore, conditioned medium obtained from the 3 bones decreased alkaline phosphatase activity in MC3T3-E1 osteogenic cells. These preliminary data demonstrate that particulated bone grafts, regardless of embryological origin, mode of ossification and morphology, release a similar TGF-β1 activity.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1049-2275

Publisher:

Lippincott Williams & Wilkins

Language:

English

Submitter:

Doris Burri

Date Deposited:

18 Jul 2019 14:27

Last Modified:

13 Mar 2021 13:40

Publisher DOI:

10.1097/SCS.0000000000004251

PubMed ID:

29381614

BORIS DOI:

10.7892/boris.125243

URI:

https://boris.unibe.ch/id/eprint/125243

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