Antimicrobial influence of different root canal filling techniques in experimentally infected human root canals.

Tennert, Christian; Schurig, Tilman; Al-Ahmad, Ali; Strobel, Sabrina Lydia; Kielbassa, Andrej M; Wrbas, Karl-Thomas (2017). Antimicrobial influence of different root canal filling techniques in experimentally infected human root canals. Quintessence international, 48(4), pp. 273-280. Quintessenz Verlags-GmbH 10.3290/j.qi.a37129

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OBJECTIVE The aim of the present study was to evaluate the antimicrobial influence of different root canal filling techniques using gutta-percha and an epoxy resin-based sealer in experimentally infected root canals of extracted human teeth. METHOD AND MATERIALS In total, 96 intact sterilized, permanent human anterior teeth and premolars with single patent root canals were prepared and infected with a clinical isolate of Enterococcus faecalis. After 72 hours, all root canals were sampled using three sterile paper points. The tooth specimens were randomly divided into three groups and a control of 24 specimens each, according to the respective obturation techniques: lateral condensation (LC group), ProTaper Thermafil (PT group), and vertical compaction technique (VC group). AH Plus was used as sealer. The control group was left untreated (without root canal filling). After 7 days root canal fillings were removed and collected. The root canals were sampled using three sterile paper points and dentin chips were obtained from the root canal walls. The samples were cultured on blood agar, and colony forming units were counted. RESULTS All root canal filling techniques significantly reduced bacterial viability, eliminating more than 99.9% of E faecalis. In the LC group, three (13%) root canals were culture negative. In the PT group, 21 (88%) root canals and in the VC group 15 (54%) were culture negative. CONCLUSION All root canal filling techniques significantly reduced E faecalis in root canals. In cases where warm filling techniques can be applied, these should be preferred to cold obturation.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Tennert, Christian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0033-6572

Publisher:

Quintessenz Verlags-GmbH

Language:

English

Submitter:

Hendrik Meyer-Lückel

Date Deposited:

31 Jul 2019 08:39

Last Modified:

31 Jul 2019 08:39

Publisher DOI:

10.3290/j.qi.a37129

PubMed ID:

27834415

BORIS DOI:

10.7892/boris.125266

URI:

https://boris.unibe.ch/id/eprint/125266

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