Proteomics of acquired pellicle in gastroesophageal reflux disease patients with or without erosive tooth wear.

Martini, Tatiana; Rios, Daniela; Cassiano, Luiza Paula Silva; Silva, Cíntia Maria de Souza; Taira, Even Akemi; Ventura, Talita Mendes Silva; Pereira, Heloísa Aparecida Barbosa Silva; Magalhães, Ana Carolina; Saads Carvalho, Thiago; Baumann, Tommy; Lussi, Adrian; Oliveira, Ricardo Brandt; Palma-Dibb, Regina Guenka; Buzalaf, Marília Afonso Rabelo (2019). Proteomics of acquired pellicle in gastroesophageal reflux disease patients with or without erosive tooth wear. Journal of dentistry, 81, pp. 64-69. Elsevier Science 10.1016/j.jdent.2018.12.007

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This in vivo study compared the protein profile of the acquired enamel pellicle (AEP) in volunteers 1) with gastroesophageal reflux disease (GERD) and erosive tooth wear (ETW) (BEWE ≥ 9; GE group); 2) with GERD without ETW (BEWE = 0; GNE group) and 3) control (without GERD and BEWE = 0; C group).


Twenty-four subjects (8/group) participated. AEP was formed during 120 min and collected. After protein extraction, the samples were submitted to reverse phase liquid chromatography coupled to mass spectrometry. Label-free proteomic quantification was performed using Protein Lynx Global Service software.


In total, 458 proteins were identified. Seventy-six proteins were common to all the groups. The proteomic profile of the AEP was quite different among the distinct groups. The numbers of proteins exclusively found in the C, GE and GNE groups were 113, 110 and 81, respectively. Most of the proteins exclusively identified in the C and GNE groups bind metals, while those in the GE group are mainly membrane proteins. Many proteins were found exclusively in the reflux groups. In the quantitative analyses, when the GNE group was compared with the GE group, the proteins with the highest decreases were Lysozyme C, Antileukoproteinase, Cathepsin G, Neutrophil defensins and Basic salivary proline-rich proteins, while those with the highest increases were subunits of Hemoglobin, Albumin and isoforms of Cystatin.


Profound alterations in the proteomic profile of the AEP were seen in GNE compared with GE volunteers, which might play a role in the resistance to ETW seen in the first.


This pioneer study compared the proteomic profile of the AEP of patients with GERD with or without ETW. Increased proteins in those without ETW might be protective and are good candidates to be added to dental products to protect against erosion caused by intrinsic acids.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry

UniBE Contributor:

Saads Carvalho, Thiago, Baumann, Tommy, Lussi, Adrian


600 Technology > 610 Medicine & health




Elsevier Science




Hendrik Meyer-Lückel

Date Deposited:

12 Apr 2019 16:06

Last Modified:

05 Dec 2022 15:25

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Acquired enamel pellicle Erosive tooth wear Gastroesophageal reflux Proteomics




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