Bone-conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model.

Kuchler, Ulrike; Rybaczek, Tina; Dobask, Toni; Heimel, Patrick; Tangl, Stefan; Klehm, Jessica; Menzel, Matthias; Gruber, Reinhard (2018). Bone-conditioned medium modulates the osteoconductive properties of collagen membranes in a rat calvaria defect model. Clinical oral implants research, 29(4), pp. 381-388. Wiley-Blackwell 10.1111/clr.13133

[img] Text
Kuchler_et_al-2018-Clinical_Oral_Implants_Research.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

OBJECTIVES Collagen membranes are not limited to be occlusive barriers as they actively support bone regeneration. However, the impact of bone-derived growth factors on their osteoconductive competence has not been examined. METHODS Twenty adult Sprague Dawley rats were included in the study. Calvaria defects with a diameter of five millimeter were created. The defect was covered with one layer of a collagen membrane previously soaked in conditioned medium of porcine bone chips or in culture medium alone. After 4 weeks, microcomputed tomography was performed. Undecalcified thin-ground sections were subjected to light and scanning electron microscopy. Primary outcome parameter was the bone volume in the defect. Unit of analysis was the bone-conditioned medium (BCM). RESULTS In the central defect area of the control and the BCM group, median new bone connected to the host bone was 0.54 and 0.32 mm³, respectively (p = .10). In the ectocranial defect area, the control group showed significantly more bone than the BCM group (0.90 and 0.26 mm³; p = .02). Based on an exploratory interpretation, the control group had smaller bony islands than the BCM group. Scanning electron microscopy and histology indicate the formation of bone but also the collagen membrane to be mineralized in the defect site. CONCLUSIONS These results demonstrate that the commercial collagen membrane holds an osteoconductive competence in a rat calvaria defect model. Soaking collagen membranes with BCM shifts bone formation toward the formation of bony islands rather than new bone connected to the host bone.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Doris Burri

Date Deposited:

03 Jul 2019 11:24

Last Modified:

26 Oct 2019 22:04

Publisher DOI:

10.1111/clr.13133

PubMed ID:

29453780

Uncontrolled Keywords:

bone conditioned medium calvaria defect membranes morphometry mouse osteoconductivity

BORIS DOI:

10.7892/boris.125344

URI:

https://boris.unibe.ch/id/eprint/125344

Actions (login required)

Edit item Edit item
Provide Feedback