Healing of two-wall intra-bony defects treated with a novel EMD-liquid-A pre-clinical study in monkeys.

Shirakata, Yoshinori; Miron, Richard John; Shinohara, Yukiya; Nakamura, Toshiaki; Sena, Kotaro; Horai, Naoto; Bosshardt, Dieter; Noguchi, Kazuyuki; Sculean, Anton (2017). Healing of two-wall intra-bony defects treated with a novel EMD-liquid-A pre-clinical study in monkeys. Journal of clinical periodontology, 44(12), pp. 1264-1273. Wiley 10.1111/jcpe.12825

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AIM To investigate the effect of a novel enamel matrix derivative formulation (EMD-liquid or Osteogain) combined with an absorbable collagen sponge (ACS) on periodontal wound healing in intra-bony defects in monkeys. MATERIALS AND METHODS Chronic two-wall intra-bony defects were created at the distal aspect of eight teeth in three monkeys (Macaca fascicularis). The 24 defects were randomly assigned to one of the following treatments: (i) open flap debridement (OFD) + ACS alone, (ii) OFD + Emdogain + ACS (Emdogain/ACS), (iii) OFD + Osteogain + ACS (Osteogain/ACS) or (iv) OFD alone. At 4 months, the animals were euthanized for histologic evaluation. RESULTS Osteogain/ACS resulted in more consistent formation of cementum, periodontal ligament and bone with limited epithelial proliferation compared to OFD alone, Emdogain/ACS and OFD + ACS. Among the four treatment groups, the Osteogain/ACS group demonstrated the highest amount of regenerated tissues. However, complete periodontal regeneration was not observed in any of the defects in the four groups. CONCLUSIONS The present findings indicate that in two-wall intra-bony defects, reconstructive surgery with Osteogain/ACS appears to be a promising novel approach for facilitating periodontal wound healing/regeneration, thus warranting further clinical testing.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Shirakata, Yoshinori; Miron, Richard John; Bosshardt, Dieter and Sculean, Anton


600 Technology > 610 Medicine & health








Doris Burri

Date Deposited:

31 Jul 2019 07:51

Last Modified:

22 Oct 2019 16:42

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

animal study carrier enamel matrix proteins intra-bony defect periodontal regeneration





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