Nd:YAG laser radiation (1.064 nm) accelerates differentiation of osteoblasts to osteocytes on smooth and rough titanium surfaces in vitro.

Karoussis, Ioannis K; Kyriakidou, Kyriaki; Psarros, Costas; Lang, Niklaus Peter; Vrotsos, Ioannis A (2017). Nd:YAG laser radiation (1.064 nm) accelerates differentiation of osteoblasts to osteocytes on smooth and rough titanium surfaces in vitro. Clinical oral implants research, 28(7), pp. 785-790. Wiley-Blackwell 10.1111/clr.12882

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BACKGROUND A number of studies revealed beneficial effects of low-level laser therapy (LLLT) regarding cell proliferation and differentiation. AIM To investigate the effect of Nd:YAG (1.064 nm) laser radiation in the proliferation and differentiation potential of MG-63 osteoblast-like cells. Additionally, the effects of the surface configurations were to be evaluated. MATERIAL AND METHODS MG-63 osteoblast cells were cultured on different surfaces: plastic tissue culture, smooth (polished) titanium-PT and rough titanium-SLA. The effects of both titanium surfaces and low-level laser therapy (LLLT) on cell adhesion were evaluated by the gene expression of molecules involved in cell proliferation and differentiation. In addition, scanning electron microscopy (SEM) and MTT proliferation assays were used to examine cell morphology and proliferation, respectively. RESULTS Compared to smooth (PT) surfaces, SLA surfaces favoured MG-63 cell differentiation. Following the application of Nd:YAG laser irradiation, cells yielded statistically significantly improved differentiation on both smooth and SLA surfaces compared with non-irradiated surfaces. CONCLUSIONS The findings of this present study suggest that both surface morphology and Nd:YAG laser irradiation influence the proliferation and differentiation potential of MG-63 cells.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Lang, Niklaus Peter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0905-7161

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Doris Burri

Date Deposited:

26 Jul 2019 16:29

Last Modified:

24 Oct 2019 03:49

Publisher DOI:

10.1111/clr.12882

PubMed ID:

27279385

Uncontrolled Keywords:

SLA low-level laser therapy MG-63 osteoblasts Nd:YAG laser differentiation gene expression scanning electron microscopy titanium surfaces

BORIS DOI:

10.7892/boris.125425

URI:

https://boris.unibe.ch/id/eprint/125425

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