Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures.

Abd El Hafez, Mai Moustafa Ahmed; Shaw-Boden, Jane; Wilbs, Jonas; Despont, Alain; Rieben, Robert (2018). Improvement of a Closed Chest Porcine Myocardial Infarction Model by Standardization of Tissue and Blood Sampling Procedures. Journal of visualized experiments(133) MYJoVE Corporation 10.3791/56856

[img] Text
document(2).pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Myocardial ischemia reperfusion (I/R) injury contributes to almost half of the necrotic area after myocardial infarction. To date there is no approved drug to prevent or reduce myocardial I/R injury. The study and understanding of the pathophysiological mechanisms of myocardial I/R injury is essential to develop successful treatments. Large animal experiments are an important step in translational methods. The porcine model of acute myocardial infarction has been established and described by ourselves and others. We aimed to further improve the value of the model by focusing in detail on the sampling techniques for use in future experiments. Furthermore, we emphasize small but important steps that can affect the quality of the final results. To mimic the clinical situation of myocardial I/R injury, a percutaneous coronary intervention (PCI) catheter was inserted into the left anterior descending coronary artery (LAD) of an anesthetized pig. °°°This model mimics acute myocardial infarction and PCI treatment in humans with the possibility of accurately determining the area at risk as well as the necrotic- and viable ischemic tissue. Here the model was used to investigate the effect of a bicyclic peptide inhibitor of FXIIa. The model can also be modified to allow longer reperfusion times to study later effects of myocardial infarction.

Item Type:

Journal Article (Further Contribution)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Abd El Hafez, Mai Moustafa Ahmed; Shaw-Boden, Jane; Despont, Alain and Rieben, Robert

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-087X

Publisher:

MYJoVE Corporation

Language:

English

Submitter:

Marla Rittiner

Date Deposited:

27 Feb 2019 14:48

Last Modified:

27 Feb 2019 14:48

Publisher DOI:

10.3791/56856

PubMed ID:

29578502

BORIS DOI:

10.7892/boris.125551

URI:

https://boris.unibe.ch/id/eprint/125551

Actions (login required)

Edit item Edit item
Provide Feedback