Longitudinal investigation of the metabolome of 3D aggregating brain cell cultures at different maturation stages by H HR-MAS NMR.

Diserens, Gaëlle; Vermathen, Martina; Zurich, Marie-Gabrielle; Vermathen, Peter (2018). Longitudinal investigation of the metabolome of 3D aggregating brain cell cultures at different maturation stages by H HR-MAS NMR. Analytical and bioanalytical chemistry, 410(26), pp. 6733-6749. Springer 10.1007/s00216-018-1295-0

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The aim of the present study was to establish the developmental profile of metabolic changes of 3D aggregating brain cell cultures by H high-resolution magic angle spinning (HR-MAS) NMR spectroscopy. The histotypic 3D brain aggregate, containing all brain cell types, is an excellent model for mechanistic studies including OMICS analysis; however, their metabolic profile has not been yet fully investigated. Chemometric analysis revealed a clear separation of samples from the different maturation time points. Metabolite concentration evolutions could be followed and revealed strong and various metabolic alterations. The strong metabolite evolution emphasizes the brain modeling complexity during maturation, possibly reflecting physiological processes of brain tissue development. The small observed intra- and inter-experimental variabilities show the robustness of the combination of H-HR-MAS NMR and 3D brain aggregates, making it useful to investigate mechanisms of toxicity that will ultimately contribute to improve predictive neurotoxicology. Graphical Abstract ᅟ.

Item Type:

Journal Article (Original Article)


08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
04 Faculty of Medicine > Department of Radiology, Neuroradiology and Nuclear Medicine (DRNN) > Institute of Diagnostic, Interventional and Paediatric Radiology > DCR Magnetic Resonance Spectroscopy and Methodology (AMSM)

UniBE Contributor:

Diserens, Gaëlle; Vermathen, Martina and Vermathen, Peter


500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry








Isabelle Ursula Saurer

Date Deposited:

13 Mar 2019 10:55

Last Modified:

23 Oct 2019 11:29

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Uncontrolled Keywords:

3D brain cell cultures Brain aggregates Chemometrics High-resolution magic angle spinning Nuclear magnetic resonance





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