An SSP-PCR method for the rapid detection of disease-associated alleles HLA-A*29 and HLA-B*51.

Amstutz, Ursula; Schärer, Dominic Manuel; Andrey, G; Wirthmüller, Urs; Largiadèr, Carlo Rodolfo (2018). An SSP-PCR method for the rapid detection of disease-associated alleles HLA-A*29 and HLA-B*51. HLA : immune response genetic, 92(2), pp. 90-93. Wiley 10.1111/tan.13296

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HLA-A*29 and HLA-B*51 are associated with birdshot uveitis and Behçet's disease, respectively, and are used as a diagnostic criterion in patients with suspected disease, requiring their detection in diagnostic laboratories. While commercial tests for individual HLA alleles are available for other disease-associated HLA variants, no similar allele-specific assays are available for HLA-A*29 and HLA-B*51. Here, we report sequence-specific priming-polymerase chain reaction (SSP-PCR) methods for the detection of HLA-A*29 and HLA-B*51 using a single PCR reaction per allele. The assays were tested in 30 and 32 previously HLA-typed samples, respectively, representing >97% of HLA-A alleles and >93% of HLA-B alleles in a European population. A concordance of 100% was observed with previous typing results, validating these methods for use in a diagnostic or research context.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry

UniBE Contributor:

Amstutz, Ursula; Schärer, Dominic Manuel; Wirthmüller, Urs and Largiadèr, Carlo Rodolfo

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2059-2310

Publisher:

Wiley

Language:

English

Submitter:

Marie-Christine Müller

Date Deposited:

02 Apr 2019 14:16

Last Modified:

29 Oct 2019 02:51

Publisher DOI:

10.1111/tan.13296

PubMed ID:

29766667

Uncontrolled Keywords:

HLA-A*29 HLA-B*51 HLA SSP-PCR disease association

BORIS DOI:

10.7892/boris.125803

URI:

https://boris.unibe.ch/id/eprint/125803

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