Influence of centrifugation conditions on the results of 77 routine clinical chemistry analytes using standard vacuum blood collection tubes and the new BD-Barricor tubes

Cadamuro, Janne; Mrazek, Cornelia; Leichtle, Alexander Benedikt; Kipman, Ulrike; Felder, Thomas K.; Wiedemann, Helmut; Oberkofler, Hannes; Fiedler, Georg Martin; Haschke-Becher, Elisabeth (2018). Influence of centrifugation conditions on the results of 77 routine clinical chemistry analytes using standard vacuum blood collection tubes and the new BD-Barricor tubes. Biochemia Medica, 28(1), 010704. Croatian Society of Medical Biochemistry and Laboratory Medicine 10.11613/BM.2018.010704

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Introduction: Although centrifugation is performed in almost every blood sample, recommendations on duration and g-force are heterogeneo-us and mostly based on expert opinions. In order to unify this step in a fully automated laboratory, we aimed to evaluate different centrifugation settings and their influence on the results of routine clinical chemistry analytes. Materials and methods: We collected blood from 41 healthy volunteers into BD Vacutainer PST II-heparin-gel- (LiHepGel), BD Vacutainer SST II-serum-, and BD Vacutainer Barricor heparin-tubes with a mechanical separator (LiHepBar). Tubes were centrifuged at 2000xg for 10 minutes and 3000xg for 7 and 5 minutes, respectively. Subsequently 60 and 21 clinical chemistry analytes were measured in plasma and serum samples, respec-tively, using a Roche COBAS instrument. Results: High sensitive Troponin T, pregnancy-associated plasma protein A, ß human chorionic gonadotropin and rheumatoid factor had to be excluded from statistical evaluation as many of the respective results were below the measuring range. Except of free haemoglobin (fHb) measure-ments, no analyte result was altered by the use of shorter centrifugation times at higher g-forces. Comparing LiHepBar to LiHepGel tubes at different centrifugation setting, we found higher lactate-dehydrogenase (LD) (P = 0.003 to < 0.001) and lower bicarbonate values (P = 0.049 to 0.008) in the lat ter. Conclusions: Serum and heparin samples may be centrifuged at higher speed (3000xg) for a shorter amount of time (5 minutes) without alteration of the analytes tested in this study. When using LiHepBar tubes for blood collection, a separate LD reference value might be needed.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Institute of Clinical Chemistry

UniBE Contributor:

Leichtle, Alexander Benedikt and Fiedler, Georg Martin

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1330-0962

Publisher:

Croatian Society of Medical Biochemistry and Laboratory Medicine

Language:

English

Submitter:

Marie-Christine Müller

Date Deposited:

02 Apr 2019 09:48

Last Modified:

07 Apr 2019 02:38

Publisher DOI:

10.11613/BM.2018.010704

PubMed ID:

29187797

BORIS DOI:

10.7892/boris.125821

URI:

https://boris.unibe.ch/id/eprint/125821

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