Fungal resistance mediated by maize wall-associated kinase ZmWAK-RLK1 correlates with reduced benzoxazinoid content

Yang, Ping; Praz, Coraline; Li, Beibei; Singla, Jyoti; Robert, Christelle A. M.; Kessel, Bettina; Scheuermann, Daniela; Lüthi, Linda; Ouzunova, Milena; Erb, Matthias; Krattinger, Simon G.; Keller, Beat (2019). Fungal resistance mediated by maize wall-associated kinase ZmWAK-RLK1 correlates with reduced benzoxazinoid content. New Phytologist, 221(2), pp. 976-987. Wiley-Blackwell 10.1111/nph.15419

[img] Text
2019_NewPhytol_221_976.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Wall‐associated kinases (WAKs) have recently been identified as major components of fungal and bacterial disease resistance in several cereal crop species. However, the molecular mechanisms of WAK‐mediated resistance remain largely unknown. Here, we investigated the function of the maize gene ZmWAK‐RLK1 (Htn1) that confers quantitative resistance to northern corn leaf blight (NCLB) caused by the hemibiotrophic fungal pathogen Exserohilum turcicum. ZmWAK‐RLK1 was found to localize to the plasma membrane and its presence resulted in a modification of the infection process by reducing pathogen penetration into host tissues. A large‐scale transcriptome analysis of near‐isogenic lines (NILs) differing for ZmWAK‐RLK1 revealed that several differentially expressed genes are involved in the biosynthesis of the secondary metabolites benzoxazinoids (BXs). The contents of several BXs including DIM2BOA‐Glc were significantly lower when ZmWAK‐RLK1 is present. DIM2BOA‐Glc concentration was significantly elevated in ZmWAK‐RLK1 mutants with compromised NCLB resistance. Maize mutants that were affected in overall BXs biosynthesis or content of DIM2BOA‐Glc showed increased NCLB resistance. We conclude that Htn1‐mediated NCLB resistance is associated with a reduction of BX secondary metabolites. These findings suggest a link between WAK‐mediated quantitative disease resistance and changes in biochemical fluxes starting with indole‐3‐glycerol phosphate.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Li, Beibei; Robert, Christelle Aurélie Maud and Erb, Matthias

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0028-646X

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

08 Mar 2019 16:17

Last Modified:

08 Mar 2019 16:17

Publisher DOI:

10.1111/nph.15419

PubMed ID:

30178602

Uncontrolled Keywords:

benzoxazinoids (BXs); Htn1; maize disease resistance; receptor‐like kinase; wall‐associated kinase (WAK)

BORIS DOI:

10.7892/boris.125942

URI:

https://boris.unibe.ch/id/eprint/125942

Actions (login required)

Edit item Edit item
Provide Feedback