Activity of two hyaluronan preparations on primary human oral fibroblasts

Asparuhova, Mariya B.; Kiryak, Deniz; Eliezer, Meizi; Mihov, Deyan; Sculean, Anton (2019). Activity of two hyaluronan preparations on primary human oral fibroblasts. Journal of periodontal research, 54(1), pp. 33-45. Wiley-Blackwell 10.1111/jre.12602

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BACKGROUND AND OBJECTIVE: The potential benefit of using hyaluronan (HA) in reconstructive periodontal surgery is still a matter of debate. The aim of the present study was to evaluate the effects of two HA formulations on human oral fibroblasts involved in soft tissue wound healing/regeneration. MATERIAL AND METHODS: Metabolic, proliferative and migratory abilities of primary human palatal and gingival fibroblasts were examined upon HA treatment. To uncover the mechanisms whereby HA influences cellular behavior, wound healing-related gene expression and activation of signaling kinases were analyzed by qRT-PCR and immunoblotting, respectively. RESULTS: The investigated HA formulations maintained the viability of oral fibroblasts and increased their proliferative and migratory abilities. They enhanced expression of genes encoding type III collagen and transforming growth factor-β3, characteristic of scarless wound healing. The HAs upregulated the expression of genes encoding pro-proliferative, pro-migratory, and pro-inflammatory factors, with only a moderate effect on the latter in gingival fibroblasts. In palatal but not gingival fibroblasts, an indirect effect of HA on the expression of matrix metalloproteinases 2 and 3 was detected, potentially exerted through induction of pro-inflammatory cytokines. Finally, our data pointed on Akt, Erk1/2 and p38 as the signaling molecules whereby the HAs exert their effects on oral fibroblasts. CONCLUSION: Both investigated HA formulations are biocompatible and enhance the proliferative, migratory and wound healing properties of cell types involved in soft tissue wound healing following regenerative periodontal surgery. Our data further suggest that in gingival tissues, the HAs are not likely to impair the healing process by prolonging inflammation or causing excessive MMP expression at the repair site.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine

UniBE Contributor:

Asparuhova, Mariya Bozhidarova; Eliezer-Shatz, Meizi and Sculean, Anton


600 Technology > 610 Medicine & health








Caroline Balz

Date Deposited:

05 Apr 2019 11:28

Last Modified:

24 Oct 2019 22:07

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

gene expression growth factors hyaluronic acid oral soft tissue wound healing pro-inflammatory cytokines




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