Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro.

Liljander, Anne; Sacchini, Flavio; Stoffel, Michael Hubert; Schieck, Elise; Stokar von Neuforn, Nadine; Labroussaa, Fabien; Heller, Martin; Salt, Jeremy; Frey, Joachim; Falquet, Laurent; Goovaerts, Danny; Jores, Jörg (2019). Reproduction of contagious caprine pleuropneumonia reveals the ability of convalescent sera to reduce hydrogen peroxide production in vitro. Veterinary research, 50(1), p. 10. BioMed Central 10.1186/s13567-019-0628-0

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Contagious caprine pleuropneumonia (CCPP), caused by Mycoplasma capricolum subsp. capripneumoniae is a severe disease widespread in Africa and Asia. Limited knowledge is available on the pathogenesis of this organism, mainly due to the lack of a robust in vivo challenge model and the means to do site-directed mutagenesis. This work describes the establishment of a novel caprine challenge model for CCPP that resulted in 100% morbidity using a combination of repeated intranasal spray infection followed by a single transtracheal infection employing the recent Kenyan outbreak strain ILRI181. Diseased animals displayed CCPP-related pathology and the bacteria could subsequently be isolated from pleural exudates and lung tissues in concentrations of up to 10 bacteria per mL as well as in the trachea using immunohistochemistry. Reannotation of the genome sequence of ILRI181 and F38 revealed the existence of genes encoding the complete glycerol uptake and metabolic pathways involved in hydrogen peroxide (HO) production in the phylogenetically related pathogen M. mycoides subsp. mycoides. Furthermore, the expression of L-α-glycerophosphate oxidase (GlpO) in vivo was confirmed. In addition, the function of the glycerol metabolism was verified by measurement of production of HO in medium containing physiological serum concentrations of glycerol. Peroxide production could be inhibited with serum from convalescent animals. These results will pave the way for a better understanding of host-pathogen interactions during CCPP and subsequent vaccine development.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Stoffel, Michael Hubert; Stokar von Neuforn, Nadine; Labroussaa, Fabien; Frey, Joachim and Jores, Jörg

Subjects:

600 Technology > 630 Agriculture

ISSN:

1297-9716

Publisher:

BioMed Central

Language:

English

Submitter:

Vanessa Alice Blum

Date Deposited:

08 Apr 2019 18:01

Last Modified:

14 Apr 2019 02:34

Publisher DOI:

10.1186/s13567-019-0628-0

PubMed ID:

30736863

BORIS DOI:

10.7892/boris.126553

URI:

https://boris.unibe.ch/id/eprint/126553

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