Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei.

Florini, Francesca; Naguleswaran, Arunasalam; Gharib, Walid H; Bringaud, Frédéric; Roditi, Isabel (2019). Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei. Nucleic acids research, 47(4), pp. 1725-1739. Oxford University Press 10.1093/nar/gky1255

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The path from DNA to RNA to protein in eukaryotes is guided by a series of factors linking transcription, mRNA export and translation. Many of these are conserved from yeast to humans. Trypanosomatids, which diverged early in the eukaryotic lineage, exhibit unusual features such as polycistronic transcription and trans-splicing of all messenger RNAs. They possess basal transcription factors, but lack recognisable orthologues of many factors required for transcription elongation and mRNA export. We show that retrotransposon hotspot (RHS) proteins fulfil some of these functions and that their depletion globally impairs nascent RNA synthesis by RNA polymerase II. Three sub-families are part of a coordinated process in which RHS6 is most closely associated with chromatin, RHS4 is part of the Pol II complex and RHS2 connects transcription with the translation machinery. In summary, our results show that the components of eukaryotic transcription are far from being universal, and reveal unsuspected plasticity in the course of evolution.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Florini, Francesca; Naguleswaran, Arunasalam and Roditi, Isabel

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0305-1048

Publisher:

Oxford University Press

Language:

English

Submitter:

Francesca Florini

Date Deposited:

29 May 2019 15:30

Last Modified:

25 Oct 2019 15:45

Publisher DOI:

10.1093/nar/gky1255

PubMed ID:

30544263

BORIS DOI:

10.7892/boris.126711

URI:

https://boris.unibe.ch/id/eprint/126711

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