Measurement of Contractility and Calcium Release in Cardiac Spheroids.

Zuppinger, Christian (2019). Measurement of Contractility and Calcium Release in Cardiac Spheroids. In: Heizmann, Claus W. (ed.) Calcium-Binding Proteins of the EF-Hand Superfamily. Methods in Molecular Biology: Vol. 1929 (pp. 41-52). Humana Press, New York, NY 10.1007/978-1-4939-9030-6_4

Text Measurement in cardiac spheroids accepted.pdf - Accepted Version Restricted to registered users only Available under License Publisher holds Copyright. Download (4MB)

There is a need for organotypic in vitro models that resemble the native tissue in functionality and tissue architecture for disease models and drug development. To this end, many 3D culture formats have been developed over time. Among the most often used type is the scaffold-free multicellular aggregate, also called spheroid, that forms by self-assembly. However, working with 3D cultures can be challenging because single cells are not as accessible as in 2D cultures and standard lab procedures must be adapted or replaced altogether. This chapter describes methods to create cardiac spheroids consisting of human iPSC-derived cardiomyocytes and cardiac fibroblasts and how to measure contractility or calcium signals using quantitative video analysis and confocal microscopy. Emphasis is on the particular challenges that 3D cultures pose and on affordable methods that do not require specialized equipment.

Interest & Impact

Downloads

2 since deposited on 08 Apr 2019
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item