Measurement of Contractility and Calcium Release in Cardiac Spheroids.

Zuppinger, Christian (2019). Measurement of Contractility and Calcium Release in Cardiac Spheroids. In: Heizmann, Claus W. (ed.) Calcium-Binding Proteins of the EF-Hand Superfamily. Methods in Molecular Biology: Vol. 1929 (pp. 41-52). Humana Press, New York, NY 10.1007/978-1-4939-9030-6_4

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There is a need for organotypic in vitro models that resemble the native tissue in functionality and tissue architecture for disease models and drug development. To this end, many 3D culture formats have been developed over time. Among the most often used type is the scaffold-free multicellular aggregate, also called spheroid, that forms by self-assembly. However, working with 3D cultures can be challenging because single cells are not as accessible as in 2D cultures and standard lab procedures must be adapted or replaced altogether. This chapter describes methods to create cardiac spheroids consisting of human iPSC-derived cardiomyocytes and cardiac fibroblasts and how to measure contractility or calcium signals using quantitative video analysis and confocal microscopy. Emphasis is on the particular challenges that 3D cultures pose and on affordable methods that do not require specialized equipment.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Department of Cardiovascular Disorders (DHGE) > Clinic of Cardiology

UniBE Contributor:

Zuppinger, Christian

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1940-6029

Series:

Methods in Molecular Biology

Publisher:

Humana Press, New York, NY

Funders:

[116] Swiss Heart Foundation = Schweizerische Herzstiftung

Language:

English

Submitter:

Christian Zuppinger

Date Deposited:

08 Apr 2019 18:04

Last Modified:

05 Dec 2022 15:26

Publisher DOI:

10.1007/978-1-4939-9030-6_4

PubMed ID:

30710266

Uncontrolled Keywords:

3D culture Beating Calcium Cardiomyocytes Confocal microscopy Contractility Image processing Microtissue Spheroid Video

BORIS DOI:

10.7892/boris.126724

URI:

https://boris.unibe.ch/id/eprint/126724

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