Interactions Among Host–Parasite MicroRNAs During Nosema ceranae Proliferation in Apis mellifera

Evans, Jay D.; Huang, Qiang (2018). Interactions Among Host–Parasite MicroRNAs During Nosema ceranae Proliferation in Apis mellifera. Frontiers in Microbiology, 9, p. 698. Frontiers 10.3389/fmicb.2018.00698

[img]
Preview
Text
Interactions Among Host–Parasite MicroRNAs During Nosema ceranae Proliferation in Apis mellifera.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (2MB) | Preview

We previously identified microRNA (miRNA) from Nosema ceranae and found that knockdowns of transcripts for the parasite protein Dicer greatly reduce parasite reproduction. In order to study parasitic miRNA functions and identify candidate target genes, we fed honey bees infected with N. ceranae with small interfering RNA (siRNA) targeting the N. ceranae gene Dicer. We then deep-sequenced honey bee and N. ceranae miRNAs daily across a full 6-day proliferation cycle. We found seven honey bee and five N. ceranae miRNAs that were significantly differently expressed between the infection and siRNA-Dicer groups. N. ceranae miRNA showed potentially strong impacts on the N. ceranae transcriptome, where over 79% of the total protein coding genes were significantly correlated with one or more miRNAs. N. ceranae miRNAs also can regulate honey bee metabolism and immune response, given parasitic miRNAs were secreted into the cytoplasm. Our results suggest that N. ceranae miRNAs regulate both parasite and host gene expression, providing new insights for microsporidia parasitism evolution.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Bee Health

UniBE Contributor:

Huang, Qiang

Subjects:

500 Science > 590 Animals (Zoology)
600 Technology > 630 Agriculture

ISSN:

1664-302X

Publisher:

Frontiers

Language:

English

Submitter:

Anna Papach

Date Deposited:

21 May 2019 12:48

Last Modified:

05 Sep 2020 02:59

Publisher DOI:

10.3389/fmicb.2018.00698

PubMed ID:

29692768

BORIS DOI:

10.7892/boris.127012

URI:

https://boris.unibe.ch/id/eprint/127012

Actions (login required)

Edit item Edit item
Provide Feedback