Characterization of a multi-epitope peptide with selective MHC-binding capabilities encapsulated in PLGA nanoparticles as a novel vaccine candidate against Toxoplasma gondii infection.

Roozbehani, Mona; Falak, Reza; Mohammadi, Mohsen; Hemphill, Andrew; Razmjou, Elham; Meamar, Ahmad Reza; Masoori, Leila; Khoshmirsafa, Majid; Moradi, Maryam; Gharavi, Mohammad Javad (2018). Characterization of a multi-epitope peptide with selective MHC-binding capabilities encapsulated in PLGA nanoparticles as a novel vaccine candidate against Toxoplasma gondii infection. Vaccine, 36(41), pp. 6124-6132. Elsevier 10.1016/j.vaccine.2018.08.068

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No effective human vaccine against Toxoplasma gondii (T. gondii) has yet been developed; however, a protective vaccine using immunogenic peptides in a safe delivery vehicle system offers promise. Here, we employed bioinformatics to design a multimeric recombinant T. gondii vaccine using predicted T and B cell epitopes of SAG1, AMA1, ROP2, and GRA4 proteins based on their binding capabilities to common major histocompatibility complex (MHC) molecules. Furthermore, we encapsulated the expressed protein in poly lactic-co-glycolic acid (PLGA) nanoparticles as a delivery vehicle and also used alum as an adjuvant to determine the vaccine potency of this multimeric antigen. BALB/c mice were vaccinated and then challenged with T. gondii RH strain, and the survival rate and cytokine profiles were studied. Mice vaccinated with the multi-epitope-based vaccine, both with and without PLGA, had greater Th1 immune responses, survival rates, specific antibody titers, and IFN-γ and IL-2 levels than controls, while the alum-adsorbed vaccine stimulated a Th2-type humoral immune response.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Hemphill, Andrew

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0264-410X

Publisher:

Elsevier

Language:

English

Submitter:

Andrew Hemphill

Date Deposited:

21 May 2019 14:56

Last Modified:

24 Oct 2019 12:19

Publisher DOI:

10.1016/j.vaccine.2018.08.068

PubMed ID:

30181047

Additional Information:

HOPA RPF

Uncontrolled Keywords:

Multi-epitope PLGA nanoparticle Toxoplasma gondii Vaccine

BORIS DOI:

10.7892/boris.127308

URI:

https://boris.unibe.ch/id/eprint/127308

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