Jungwirth, N; Salinas Tejedor, L; Jin, W; Gudi, V; Skripuletz, T; Stein, Veronika Maria; Tipold, A; Hoffmann, A; Stangel, M; Baumgärtner, W; Hansmann, F (2018). Mesenchymal Stem Cells Form 3D Clusters Following Intraventricular Transplantation. Journal of molecular neuroscience, 65(1), pp. 60-73. Springer 10.1007/s12031-018-1070-x
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Jungwirth2018_Article_MesenchymalStemCellsForm3DClus.pdf - Published Version Available under License Publisher holds Copyright. Download (19MB) | Preview |
Mesenchymal stem cells (MSCs) are regarded as an immune privileged cell type with numerous regeneration-promoting effects. The in vivo behavior of MSC and underlying mechanisms leading to their regenerative effects are largely unknown. The aims of this study were to comparatively investigate the in vivo behavior of canine (cMSC), human (hMSC), and murine MSC (mMSC) following intra-cerebroventricular transplantation. At 7 days post transplantation (dpt), clusters of cMSC, hMSC, and mMSC were detected within the ventricular system. At 49 dpt, cMSC-transplanted mice showed clusters mostly consisting of extracellular matrix lacking transplanted MSC. Similarly, hMSC-transplanted mice lacked MSC clusters at 49 dpt. Xenogeneic MSC transplantation was associated with a local T lymphocyte-dominated immune reaction at both time points. Interestingly, no associated inflammation was observed following syngeneic mMSC transplantation. In conclusion, transplanted MSC formed intraventricular cell clusters and exhibited a short life span in vivo. Xenogeneically in contrast to syngeneically transplanted MSC triggered a T cell-mediated graft rejection indicating that MSCs are not as immune privileged as previously assumed. However, MSC may mediate their effects by a "hit and run" mechanism and future studies will show whether syngeneically or xenogeneically transplanted MSCs exert better therapeutic effects in animals with CNS disease.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > DKV - Clinical Neurology 05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) |
UniBE Contributor: |
Stein, Veronika Maria |
Subjects: |
600 Technology > 630 Agriculture |
ISSN: |
0895-8696 |
Publisher: |
Springer |
Language: |
English |
Submitter: |
Veronika Maria Stein |
Date Deposited: |
13 May 2019 11:02 |
Last Modified: |
05 Dec 2022 15:26 |
Publisher DOI: |
10.1007/s12031-018-1070-x |
PubMed ID: |
29705933 |
BORIS DOI: |
10.7892/boris.127364 |
URI: |
https://boris.unibe.ch/id/eprint/127364 |
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