Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4.

Sautter, Carmen Alexandra Nadine; Auray, Gael; Python, Sylvie; Liniger, Matthias; Summerfield, Artur (2018). Phenotypic and functional modulations of porcine macrophages by interferons and interleukin-4. Developmental and Comparative Immunology, 84, pp. 181-192. Elsevier 10.1016/j.dci.2018.01.018

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Considering that macrophage functions are strongly impacted by the local tissue environment and the type of immune response, the aim of this study was to carefully set the methodological baseline for phenotype and functions of polarized porcine monocyte-derived macrophages. To this end, macrophages were generated in autologous serum alone or with colony-stimulating factor (CSF)-1 or CSF-2, and subsequently polarized with interferon (IFN)γ, interleukin-4 or IFNβ. IFNγ promoted expression of MHC class I, MHC class II, CD11a, and CD40 as well as LPS-induced IL-6 and IL-12. A hallmark of interleukin-4 was Arginase 1 and CD203a upregulation, without abrogating pro-inflammatory cytokine production. IFNβ induced CD169, MHC class I, CD40, CD80/86, but suppressed IL-6, IL-12 and tumor-necrosis-factor secretion. CSF-2 alone altered macrophage differentiation and promoted an IFNγ-like polarization. Altogether, the results provide a comprehensive overview of porcine macrophage polarization, and demonstrate commonalities with other species as well as peculiarities of the pig.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction

UniBE Contributor:

Sautter, Carmen Alexandra Nadine; Auray, Gael; Liniger, Matthias and Summerfield, Artur

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0145-305X

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

27 May 2019 16:22

Last Modified:

23 Oct 2019 11:54

Publisher DOI:

10.1016/j.dci.2018.01.018

PubMed ID:

29408047

Uncontrolled Keywords:

IFNβ IFNγ IL-4 Macrophages Pig Polarization

BORIS DOI:

10.7892/boris.127498

URI:

https://boris.unibe.ch/id/eprint/127498

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