Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation.

Vreman, Sandra; Auray, Gael; Savelkoul, Huub F J; Rebel, Annemarie; Summerfield, Artur; Stockhofe-Zurwieden, Norbert (2018). Neonatal porcine blood derived dendritic cell subsets show activation after TLR2 or TLR9 stimulation. Developmental and Comparative Immunology, 84, pp. 361-370. Elsevier 10.1016/j.dci.2018.03.012

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The present study investigated the innate immune response in vitro to determine porcine neonate responses with Toll-like receptor (TLR)2 ligand (Pam3Cys) or TLR9 ligand (CpG) and compared these with adults. We identified the same phenotypically defined dendritic cell (DC) subsets and DC proportions in porcine neonate and adult blood by flow cytometry, which were plasmacytoid DCs (pDCs): CD14CD4CD172aCADM1) and conventional DCs (cDCs), being further divided into a cDC1 (CD14CD4CD172aCADM1) and a cDC2 (CD14CD4CD172aCADM1) subset. With neonatal cells, the TLR2 ligand induced a stronger TNF expression in monocytes and pDCs, and a stronger CD80/86 upregulation in cDC1, when compared to adult cells. Furthermore, in neonatal mononuclear cells TLR9 ligand was more potent at inducing IL12p40 mRNA expression. These results indicate clear responses of porcine neonatal antigen presenting cells after TLR2 and TLR9 stimulation, suggesting that corresponding ligands could be promising candidates for neonatal adjuvant application.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction

UniBE Contributor:

Auray, Gael and Summerfield, Artur

Subjects:

600 Technology > 630 Agriculture
500 Science > 570 Life sciences; biology

ISSN:

0145-305X

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

27 May 2019 16:15

Last Modified:

24 Oct 2019 08:43

Publisher DOI:

10.1016/j.dci.2018.03.012

PubMed ID:

29555549

Uncontrolled Keywords:

Dendritic cell Innate immunity Neonate Porcine Toll like receptor ligand

BORIS DOI:

10.7892/boris.127500

URI:

https://boris.unibe.ch/id/eprint/127500

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