Environmental Stability and Infectivity of Hepatitis C Virus (HCV) in Different Human Body Fluids.

Pfänder, Stephanie; Helfritz, Fabian A; Siddharta, Anindya; Todt, Daniel; Behrendt, Patrick; Heyden, Julia; Riebesehl, Nina; Willmann, Wiebke; Steinmann, Joerg; Münch, Jan; Ciesek, Sandra; Steinmann, Eike (2018). Environmental Stability and Infectivity of Hepatitis C Virus (HCV) in Different Human Body Fluids. Frontiers in Microbiology, 9, p. 504. Frontiers 10.3389/fmicb.2018.00504

[img]
Preview
Text
fmicb-09-00504.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (481kB) | Preview

Hepatitis C virus (HCV) is a hepatotropic, blood-borne virus, but in up to one-third of infections of the transmission route remained unidentified. Viral genome copies of HCV have been identified in several body fluids, however, non-parental transmission upon exposure to contaminated body fluids seems to be rare. Several body fluids, e.g., tears and saliva, are renowned for their antimicrobial and antiviral properties, nevertheless, HCV stability has never been systematically analyzed in those fluids. We used state of the art infectious HCV cell culture techniques to investigate the stability of HCV in different body fluids to estimate the potential risk of transmission via patient body fluid material. In addition, we mimicked a potential contamination of HCV in tear fluid and analyzed which impact commercially available contact lens solutions might have in such a scenario. We could demonstrate that HCV remains infectious over several days in body fluids like tears, saliva, semen, and cerebrospinal fluid. Only hydrogen-peroxide contact lens solutions were able to efficiently inactivate HCV in a suspension test. These results indicate that HCV, once it is present in various body fluids of infected patients, remains infective and could potentially contribute to transmission upon direct contact.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Pfänder, Stephanie

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

1664-302X

Publisher:

Frontiers

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

21 May 2019 12:50

Last Modified:

23 Oct 2019 07:40

Publisher DOI:

10.3389/fmicb.2018.00504

PubMed ID:

29636728

Uncontrolled Keywords:

cerebrospinal fluid contact lens solution hepatitis C virus infectivity saliva semen tear

BORIS DOI:

10.7892/boris.127502

URI:

https://boris.unibe.ch/id/eprint/127502

Actions (login required)

Edit item Edit item
Provide Feedback