Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses.

Braun, Ueli; Janett, Fredi; Züblin, Sandra; von Büren, Michèle; Hilbe, Monika; Zanoni, Reto Giacomo; Schweizer, Matthias (2018). Insemination with border disease virus-infected semen results in seroconversion in cows but not persistent infection in fetuses. BMC veterinary research, 14(1), pp. 1-8. BioMed Central 10.1186/s12917-018-1472-6

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BACKGROUND This study examined various health variables in cows after artificial insemination with Border disease virus (BDV)-infected semen and the occurrence of persistent infection in ensuing fetuses. Five cows were inseminated (day 0) with BDV-infected semen as well as with semen from a fertile Eringer bull. One cow, inseminated with virus-free semen only, served as a control. Clinical examination, assessment of eating and rumination activities, measurement of intraruminal temperature and leukocyte count were used to monitor the health of the cows. Blood samples were collected at regular intervals for the detection of viral RNA and antibodies against BDV, and the cows were slaughtered on day 56. The uteri, placentae and fetuses were examined macroscopically, histologically, immunohistochemically and by means of molecular methods for the presence of pestiviruses. RESULTS The demeanour, eating and rumination activities and intraruminal temperature were not affected by insemination with BDV-infected semen, whereas the total leukocyte and lymphocyte counts dropped transiently and were significantly lower on day 6 than on day 0. Seroconversion occurred by day 28 in the five infected cows but not in the control cow. The uteri, placentae and fetuses had no macroscopic or histological lesions, and immunohistochemical examination and RT-PCR were negative for pestiviruses. CONCLUSIONS The findings showed that cows inseminated with BDV-infected semen seroconverted and fetuses thus produced were not persistently infected. Transmission of BDV to cattle through infected semen, therefore, seems to be of minor importance.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Zanoni, Reto Giacomo and Schweizer, Matthias

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

1746-6148

Publisher:

BioMed Central

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

20 May 2019 15:41

Last Modified:

20 May 2019 15:48

Publisher DOI:

10.1186/s12917-018-1472-6

PubMed ID:

29769076

Uncontrolled Keywords:

BDV Border disease virus Cattle Insemination Persistent infection Pestivirus Semen Seroconversion

BORIS DOI:

10.7892/boris.127506

URI:

https://boris.unibe.ch/id/eprint/127506

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