The shieldin complex mediates 53BP1-dependent DNA repair.

Noordermeer, Sylvie M; Adam, Salomé; Setiaputra, Dheva; Barazas, Marco; Pettitt, Stephen J; Ling, Alexanda K; Olivieri, Michele; Álvarez-Quilón, Alejandro; Moatti, Nathalie; Zimmermann, Michal; Annunziato, Stefano; Krastev, Dragomir B; Song, Feifei; Brandsma, Inger; Frankum, Jessica; Brough, Rachel; Sherker, Alana; Landry, Sébastien; Szilard, Rachel K; Munro, Meagan M; ... (2018). The shieldin complex mediates 53BP1-dependent DNA repair. Nature, 560(7716), pp. 117-121. Macmillan Journals Ltd. 10.1038/s41586-018-0340-7

[img] Text
s41586-018-0340-7.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (9MB) | Request a copy

53BP1 is a chromatin-binding protein that regulates the repair of DNA double-strand breaks by suppressing the nucleolytic resection of DNA termini. This function of 53BP1 requires interactions with PTIP and RIF1, the latter of which recruits REV7 (also known as MAD2L2) to break sites. How 53BP1-pathway proteins shield DNA ends is currently unknown, but there are two models that provide the best potential explanation of their action. In one model the 53BP1 complex strengthens the nucleosomal barrier to end-resection nucleases, and in the other 53BP1 recruits effector proteins with end-protection activity. Here we identify a 53BP1 effector complex, shieldin, that includes C20orf196 (also known as SHLD1), FAM35A (SHLD2), CTC-534A2.2 (SHLD3) and REV7. Shieldin localizes to double-strand-break sites in a 53BP1- and RIF1-dependent manner, and its SHLD2 subunit binds to single-stranded DNA via OB-fold domains that are analogous to those of RPA1 and POT1. Loss of shieldin impairs non-homologous end-joining, leads to defective immunoglobulin class switching and causes hyper-resection. Mutations in genes that encode shieldin subunits also cause resistance to poly(ADP-ribose) polymerase inhibition in BRCA1-deficient cells and tumours, owing to restoration of homologous recombination. Finally, we show that binding of single-stranded DNA by SHLD2 is critical for shieldin function, consistent with a model in which shieldin protects DNA ends to mediate 53BP1-dependent DNA repair.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Rottenberg, Sven

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health
600 Technology > 630 Agriculture

ISSN:

0028-0836

Publisher:

Macmillan Journals Ltd.

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

04 Jun 2019 15:44

Last Modified:

04 Jun 2019 15:53

Publisher DOI:

10.1038/s41586-018-0340-7

PubMed ID:

30022168

BORIS DOI:

10.7892/boris.127533

URI:

https://boris.unibe.ch/id/eprint/127533

Actions (login required)

Edit item Edit item
Provide Feedback