Archive Mining Brings to Light a 25-Year Old Astrovirus Encephalitis Case in a Sheep.

Küchler, Leonore; Koch, Michel C; Seuberlich, Torsten; Boujon, Céline L (2019). Archive Mining Brings to Light a 25-Year Old Astrovirus Encephalitis Case in a Sheep. Frontiers in veterinary science, 6, p. 51. Frontiers Media 10.3389/fvets.2019.00051

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In mammals, the small, positive-sense single-stranded RNA astroviruses are known as being mostly enterotropic and host-specific. Over the past years, however, they were identified several times in central nervous system tissues of humans, minks, cattle, sheep, and pigs with nonsuppurative inflammatory disease of that organ system. We recently reported such neurotropic astroviruses, amongst which bovine astrovirus CH15 (BoAstV-CH15) in two cows, and ovine astrovirus CH16 (OvAstV-CH16) in a sheep, which were genetically almost identical to one another. In order to investigate the occurrence of this virus species in Switzerland over time, we selected formalin-fixed, paraffin-embedded (FFPE) brain tissues of small ruminants diagnosed with severe encephalitis between 1969 and 2012 and screened those by immunohistochemistry for the capsid protein of BoAstV-CH15/OvAstV-CH16. We found one sheep, which died in 1992, that displayed positive immunostaining in various brain regions, and observed that immunostained cells were generally co-localized with the strongest histopathological lesions. We confirmed the virus presence with a second immunohistochemical protocol and demonstrated its close genetic relationship to other BoAstV-CH15/ OvAstV-CH16 strains by next-generation sequencing of an RNA extract from FFPE brain material. Our findings demonstrate that astrovirus BoAstV-CH15/OvAstV-CH16 existed in Switzerland already more than 2 decades ago and underline again the close relationship of the bovine and ovine strains of this virus.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Küchler, Leonore; Koch, Michel Christoph; Seuberlich, Torsten and Boujon, Céline Louise

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

2297-1769

Publisher:

Frontiers Media

Language:

English

Submitter:

Andrea Flükiger-Flückiger

Date Deposited:

02 Apr 2019 10:34

Last Modified:

25 Oct 2019 11:06

Publisher DOI:

10.3389/fvets.2019.00051

PubMed ID:

30886851

Uncontrolled Keywords:

astrovirus encephalitis formalin-fixed and paraffin embedded (FFPE) next-generation sequencing (NGS) sheep

BORIS DOI:

10.7892/boris.129211

URI:

https://boris.unibe.ch/id/eprint/129211

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