Multilocus sequence typing reveals clonality in Saprolegnia parasitica outbreaks.

Ravasi, Damiana; De Respinis, Sophie; Wahli, Thomas (2018). Multilocus sequence typing reveals clonality in Saprolegnia parasitica outbreaks. Journal of fish diseases, 41(11), pp. 1653-1665. Blackwell 10.1111/jfd.12869

[img] Text
Ravasi_et_al-2018-Journal_of_Fish_Diseases.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (874kB) | Request a copy

The molecular epidemiology of fish pathogen Saprolegnia parasitica is still largely unknown. We developed a multilocus sequence typing scheme based on seven housekeeping genes to characterize 77 S. parasitica strains isolated from different fish host species at different times and from different geographic areas in Switzerland between 2015 and 2017. Ten different diploid sequence types (DSTs) were identified. The majority (52%) of outbreaks in Switzerland seemed to be caused by one genotype, namely DST3, which was recovered from farm-raised and wild-caught fish in all the geographic areas and river basins included in the study. DST3 was also recovered from the rivers Bienne (eastern France) and Doubs, where the episodes of massive mortality due to saprolegniosis started in 2009. Another genotype (DST7) showed, to a lesser extent, a distribution across different river basins, while eight DSTs were unique to a defined geographic area or river basin. The occurrence of sporadic DSTs indicates a certain degree of diversity within S. parasitica in the environment. The wide distribution of DST3 suggests that a clonal population may have spread in eastern France and Switzerland across geographic barriers, with strong implications for the management of both captive and wild fish populations.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Wahli, Thomas

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0140-7775

Publisher:

Blackwell

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

05 Jun 2019 10:44

Last Modified:

24 Oct 2019 03:11

Publisher DOI:

10.1111/jfd.12869

PubMed ID:

30051543

Uncontrolled Keywords:

Saprolegnia MLST Oomycete fish diseases genotype

BORIS DOI:

10.7892/boris.129630

URI:

https://boris.unibe.ch/id/eprint/129630

Actions (login required)

Edit item Edit item
Provide Feedback