Observational Study Design in Veterinary Pathology, Part 2: Methodology.

Caswell, Jeff L; Bassel, Laura L; Rothenburger, Jamie L; Gröne, Andrea; Sargeant, Jan M; Beck, Amanda P; Ekman, Stina; Gibson-Corley, Katherine N; Kuiken, Thijs; LaDouceur, Elise E B; Meyerholz, David K; Origgi, Francesco; Posthaus, Horst; Priestnall, Simon L; Ressel, Lorenzo; Sharkey, Leslie; Teixeira, Leandro B C; Uchida, Kazuyuki; Ward, Jerrold M; Webster, Joshua D; ... (2018). Observational Study Design in Veterinary Pathology, Part 2: Methodology. Veterinary pathology, 55(6), pp. 774-785. Sage 10.1177/0300985818798121

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Observational studies are a basis for much of our knowledge of veterinary pathology, yet considerations for conducting pathology-based observational studies are not readily available. In part 1 of this series, we offered advice on planning and carrying out an observational study. Part 2 of the series focuses on methodology. Our general recommendations are to consider using already-validated methods, published guidelines, data from primary sources, and quantitative analyses. We discuss 3 common methods in pathology research-histopathologic scoring, immunohistochemistry, and polymerase chain reaction-to illustrate principles of method validation. Some aspects of quality control include use of clear objective grading criteria, validation of key reagents, assessing sample quality, determining specificity and sensitivity, use of technical and biologic negative and positive controls, blinding of investigators, approaches to minimizing operator-dependent variation, measuring technical variation, and consistency in analysis of the different study groups. We close by discussing approaches to increasing the rigor of observational studies by corroborating results with complementary methods, using sufficiently large numbers of study subjects, consideration of the data in light of similar published studies, replicating the results in a second study population, and critical analysis of the study findings.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Origgi, Francesco and Posthaus, Horst

Subjects:

600 Technology > 630 Agriculture

ISSN:

1544-2217

Publisher:

Sage

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

04 Jun 2019 16:18

Last Modified:

04 Jun 2019 16:18

Publisher DOI:

10.1177/0300985818798121

PubMed ID:

30227783

Uncontrolled Keywords:

epidemiology histologic grading immunohistochemistry method validation observational studies pathology quantitative PCR reproducibility of results research design robustness

URI:

https://boris.unibe.ch/id/eprint/129633

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