Multiplex suspension array for human anti-carbohydrate antibody profiling

Pochechueva, Tatiana; Chinarev, Alexander; Spengler, Marianne; Korchagina, Elena; Heinzelmann-Schwarz, Viola; Bovin, Nicolai; Rieben, Robert (2011). Multiplex suspension array for human anti-carbohydrate antibody profiling. Analyst, 136(3), pp. 560-9. Cambridge: Royal Society of Chemistry 10.1039/c0an00758g

Full text not available from this repository. (Request a copy)

Glycan-binding antibodies form a significant subpopulation of both natural and acquired antibodies and play an important role in various immune processes. They are for example involved in innate immune responses, cancer, autoimmune diseases, and neurological disorders. In the present study, a microsphere-based flow-cytometric immunoassay (suspension array) was applied for multiplexed detection of glycan-binding antibodies in human serum. Several approaches for immobilization of glycoconjugates onto commercially available fluorescent microspheres were compared, and as the result, the design based on coupling of end-biotinylated glycopolymers has been selected. This method requires only minute amounts of glycans, similar to a printed glycan microarray. The resulting glyco-microspheres were used for detection of IgM and IgG antibodies directed against ABO blood group antigens. The possibility of multiplexing this assay was demonstrated with mixtures of microspheres modified with six different ABO related glycans. Multiplexed detection of anti-glycan IgM and IgG correlated well with singleplex assays (Pearson's correlation coefficient r = 0.95-0.99 for sera of different blood groups). The suspension array in singleplex format for A/B trisaccharide, H(di) and Le(x) microspheres corresponded well to the standard ELISA (r > 0.94). Therefore, the described method is promising for rapid, sensitive, and reproducible detection of anti-glycan antibodies in a multiplexed format.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Herz und Gefässe

UniBE Contributor:

Rieben, Robert

ISSN:

0003-2654

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:10

Last Modified:

06 Dec 2013 13:21

Publisher DOI:

10.1039/c0an00758g

PubMed ID:

21107457

Web of Science ID:

000286326800022

URI:

https://boris.unibe.ch/id/eprint/1301 (FactScience: 202658)

Actions (login required)

Edit item Edit item
Provide Feedback