Insights into the genetic diversity of Mycobacterium tuberculosis in Tanzania.

Rutaihwa, Liliana K; Sasamalo, Mohamed; Jaleco, Aladino; Hella, Jerry; Kingazi, Ally; Kamwela, Lujeko; Kingalu, Amri; Malewo, Bryceson; Shirima, Raymond; Doetsch, Anna; Feldmann, Julia; Reinhard, Miriam; Borrell, Sonia; Brites, Daniela; Reither, Klaus; Doulla, Basra; Fenner, Lukas; Gagneux, Sebastien (2019). Insights into the genetic diversity of Mycobacterium tuberculosis in Tanzania. PLoS ONE, 14(4), e0206334. Public Library of Science 10.1371/journal.pone.0206334

[img]
Preview
Text
Rutaihwa PLoSOne 2019.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (813kB) | Preview

BACKGROUND Human tuberculosis (TB) is caused by seven phylogenetic lineages of the Mycobacterium tuberculosis complex (MTBC), Lineage 1-7. Recent advances in rapid genotyping of MTBC based on single nucleotide polymorphisms (SNP), allow for phylogenetically robust strain classification, paving the way for defining genotype-phenotype relationships in clinical settings. Such studies have revealed that, in addition to host and environmental factors, strain variation in the MTBC influences the outcome of TB infection and disease. In Tanzania, such molecular epidemiological studies of TB however are scarce in spite of a high TB burden. METHODS AND FINDINGS Here we used SNP-typing to characterize a nationwide collection of 2,039 MTBC clinical isolates representative of 1.6% of all new and retreatment TB cases notified in Tanzania during 2012 and 2013. Four lineages, namely Lineage 1-4 were identified within the study population. The distribution and frequency of these lineages varied across regions but overall, Lineage 4 was the most frequent (n = 866, 42.5%), followed by Lineage 3 (n = 681, 33.4%) and 1 (n = 336, 16.5%), with Lineage 2 being the least frequent (n = 92, 4.5%). We found Lineage 2 to be independently associated with female sex (adjusted odds ratio [aOR] 2.14; 95% confidence interval [95% CI] 1.31 - 3.50, p = 0.002) and retreatment cases (aOR 1.67; 95% CI 0.95 - 2.84, p = 0. 065) in the study population. We found no associations between MTBC lineage and patient age or HIV status. Our sublineage typing based on spacer oligotyping on a subset of Lineage 1, 3 and 4 strains revealed the presence of mainly EAI, CAS and LAM families. Finally, we detected low levels of multidrug resistant isolates among a subset of 144 retreatment cases. CONCLUSIONS This study provides novel insights into the MTBC lineages and the possible influence of pathogen-related factors on the TB epidemic in Tanzania.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine (ISPM)

UniBE Contributor:

Fenner, Lukas

Subjects:

600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services

ISSN:

1932-6203

Publisher:

Public Library of Science

Language:

English

Submitter:

Doris Kopp Heim

Date Deposited:

18 Apr 2019 12:24

Last Modified:

29 Oct 2019 14:53

Publisher DOI:

10.1371/journal.pone.0206334

PubMed ID:

30978186

Additional Information:

Fenner and Gagneux contributed equally to this work.

BORIS DOI:

10.7892/boris.130236

URI:

https://boris.unibe.ch/id/eprint/130236

Actions (login required)

Edit item Edit item
Provide Feedback