Flagellar cAMP signaling controls trypanosome progression through host tissues.

Shaw, Sebastian; DeMarco, Stephanie F; Rehmann, Ruth; Wenzler, Tanja; Florini, Francesca; Roditi, Isabel; Hill, Kent L (2019). Flagellar cAMP signaling controls trypanosome progression through host tissues. Nature communications, 10(1), p. 803. Nature Publishing Group 10.1038/s41467-019-08696-y

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The unicellular parasite Trypanosoma brucei is transmitted between mammals by tsetse flies. Following the discovery that flagellar phosphodiesterase PDEB1 is required for trypanosomes to move in response to signals in vitro (social motility), we investigated its role in tsetse flies. Here we show that PDEB1 knockout parasites exhibit subtle changes in movement, reminiscent of bacterial chemotaxis mutants. Infecting flies with the knockout, followed by live confocal microscopy of fluorescent parasites within dual-labelled insect tissues, shows that PDEB1 is important for traversal of the peritrophic matrix, which separates the midgut lumen from the ectoperitrophic space. Without PDEB1, parasites are trapped in the lumen and cannot progress through the cycle. This demonstrates that the peritrophic matrix is a barrier that must be actively overcome and that the parasite's flagellar cAMP signaling pathway facilitates this. Migration may depend on perception of chemotactic cues, which could stem from co-infecting parasites and/or the insect host.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Shaw, Sebastian; Rehmann, Ruth; Florini, Francesca and Roditi, Isabel

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

2041-1723

Publisher:

Nature Publishing Group

Language:

English

Submitter:

Isabel Roditi

Date Deposited:

02 Jul 2019 13:29

Last Modified:

23 Oct 2019 14:25

Publisher DOI:

10.1038/s41467-019-08696-y

PubMed ID:

30778051

Uncontrolled Keywords:

Trypanosome; tsetse; social motility, cyclic AMP signalling

BORIS DOI:

10.7892/boris.130288

URI:

https://boris.unibe.ch/id/eprint/130288

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