Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Diafa, Stella; Evéquoz, Damien; Leumann, Christian; Hollenstein, Marcel (2019). Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs. In: Shank, Nathaniel (ed.) Non-Natural Nucleic Acids. Methods in Molecular Biology: Vol. 1973 (pp. 1-13). New York, NY: Springer New York 10.1007/978-1-4939-9216-4_1

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Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP.

Item Type:	Book Section (Book Chapter)
Division/Institute:	08 Faculty of Science > Department of Chemistry, Biochemistry and Pharmaceutical Sciences (DCBP)
UniBE Contributor:	Diafa, Stella, Evéquoz, Damien, Leumann, Christian, Hollenstein, Marcel
Subjects:	500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry
ISBN:	978-1-4939-9216-4
Series:	Methods in Molecular Biology
Publisher:	Springer New York
Language:	English
Submitter:	Christian Leumann
Date Deposited:	22 May 2019 12:32
Last Modified:	05 Dec 2022 15:28
Publisher DOI:	10.1007/978-1-4939-9216-4_1
Related URLs:	Publisher
URI:	https://boris.unibe.ch/id/eprint/130484