Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs

Diafa, Stella; Evéquoz, Damien; Leumann, Christian; Hollenstein, Marcel (2019). Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs. In: Shank, Nathaniel (ed.) Non-Natural Nucleic Acids. Methods in Molecular Biology: Vol. 1973 (pp. 1-13). New York, NY: Springer New York 10.1007/978-1-4939-9216-4_1

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Chemical modification of nucleic acids can be achieved by the enzymatic polymerization of modified nucleoside triphosphates (dN*TPs). This approach obviates some of the requirements and drawbacks imposed by the more traditional solid-phase synthesis of oligonucleotides. Here, we describe the protocol that is necessary to synthesize dN*TPs and evaluate their substrate acceptance by polymerases for their subsequent use in various applications including selection experiments to identify aptamers. The protocol is exemplified for a sugar-constrained nucleoside analog, 7′,5′-bc-TTP.

Item Type:

Book Section (Book Chapter)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Diafa, Stella; Evéquoz, Damien; Leumann, Christian and Hollenstein, Marcel

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISBN:

978-1-4939-9216-4

Series:

Methods in Molecular Biology

Publisher:

Springer New York

Language:

English

Submitter:

Christian Leumann

Date Deposited:

22 May 2019 12:32

Last Modified:

22 May 2019 12:32

Publisher DOI:

10.1007/978-1-4939-9216-4_1

Related URLs:

URI:

https://boris.unibe.ch/id/eprint/130484

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