Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland.

Seth-Smith, Helena M B; Casanova, Carlo; Sommerstein, Rami; Meinel, Dominik M; Abdelbary, Mohamed M H; Blanc, Dominique S; Droz, Sara; Führer, Urs; Lienhard, Reto; Lang, Claudia; Dubuis, Olivier; Schlegel, Matthias; Widmer, Andreas; Keller, Peter M; Marschall, Jonas; Egli, Adrian (2019). Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland. Emerging infectious diseases, 25(6), pp. 1084-1092. U.S. National Center for Infectious Diseases 10.3201/eid2506.172119

[img] Text
17-2119.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

A recent hospital outbreak related to premoistened gloves used to wash patients exposed the difficulties of defining Burkholderia species in clinical settings. The outbreak strain displayed key B. stabilis phenotypes, including the inability to grow at 42°C; we used whole-genome sequencing to confirm the pathogen was B. stabilis. The outbreak strain genome comprises 3 chromosomes and a plasmid, sharing an average nucleotide identity of 98.4% with B. stabilis ATCC27515 BAA-67, but with 13% novel coding sequences. The genome lacks identifiable virulence factors and has no apparent increase in encoded antimicrobial drug resistance, few insertion sequences, and few pseudogenes, suggesting this outbreak was an opportunistic infection by an environmental strain not adapted to human pathogenicity. The diversity among outbreak isolates (22 from patients and 16 from washing gloves) is only 6 single-nucleotide polymorphisms, although the genome remains plastic, with large elements stochastically lost from outbreak isolates.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Infectiology

UniBE Contributor:

Sommerstein, Rami and Marschall, Jonas

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1080-6040

Publisher:

U.S. National Center for Infectious Diseases

Language:

English

Submitter:

Annelies Luginbühl

Date Deposited:

17 Jul 2019 14:23

Last Modified:

24 Oct 2019 23:39

Publisher DOI:

10.3201/eid2506.172119

PubMed ID:

31107229

Uncontrolled Keywords:

Bcc Burkholderia stabilis DNA Switzerland bacteria hospital-associated infections resistance virulence whole-genome sequencing

BORIS DOI:

10.7892/boris.131120

URI:

https://boris.unibe.ch/id/eprint/131120

Actions (login required)

Edit item Edit item
Provide Feedback