Extraocular muscle function is impaired in ryr3 -/- mice.

Eckhardt, Jan; Bachmann, Christoph; Sekulic-Jablanovic, Marijana; Enzmann, Volker; Park, Ki Ho; Ma, Jianjie; Takeshima, Hiroshi; Zorzato, Francesco; Treves, Susan (2019). Extraocular muscle function is impaired in ryr3 -/- mice. Journal of general physiology, 151(7), pp. 929-943. Rockefeller University Press 10.1085/jgp.201912333

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Calcium is an ubiquitous second messenger mediating numerous physiological processes, including muscle contraction and neuronal excitability. Ca is stored in the ER/SR and is released into the cytoplasm via the opening of intracellular inositol trisphosphate receptor and ryanodine receptor calcium channels. Whereas in skeletal muscle, isoform 1 of the RYR is the main channel mediating calcium release from the SR leading to muscle contraction, the function of ubiquitously expressed ryanodine receptor 3 (RYR3) is far from clear; it is not known whether RYR3 plays a role in excitation-contraction coupling. We recently reported that human extraocular muscles express high levels of RYR3, suggesting that such muscles may be useful to study the function of this isoform of the Ca channel. In the present investigation, we characterize the visual function of mice. We observe that ablation of RYR3 affects both mechanical properties and calcium homeostasis in extraocular muscles. These changes significantly impact vision. Our results reveal for the first time an important role for RYR3 in extraocular muscle function.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ophthalmology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Augenklinik > Forschungsgruppe Augenheilkunde

UniBE Contributor:

Enzmann, Volker

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0022-1295

Publisher:

Rockefeller University Press

Language:

English

Submitter:

Volker Enzmann

Date Deposited:

07 Aug 2019 13:48

Last Modified:

17 Dec 2019 17:04

Publisher DOI:

10.1085/jgp.201912333

PubMed ID:

31085573

BORIS DOI:

10.7892/boris.131150

URI:

https://boris.unibe.ch/id/eprint/131150

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