Fully Automated Determination of Phosphatidylethanol 16:0/18:1 and 16:0/18:2 in Dried Blood Spots.

Luginbühl, Marc Joel; Gaugler, Stefan; Weinmann, Wolfgang (2019). Fully Automated Determination of Phosphatidylethanol 16:0/18:1 and 16:0/18:2 in Dried Blood Spots. Journal of analytical toxicology, 43(6), pp. 489-496. Oxford University Press 10.1093/jat/bkz035

[img] Text
bkz035.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (534kB) | Request a copy

PURPOSE Direct alcohol markers are widely applied during abstinence monitoring, driving aptitude assessments and workplace drug testing. The most promising direct alcohol marker was found to be phosphatidylethanol (PEth). Compared to other markers it shows a long window of detection due to accumulation in blood. To facilitate and accelerate the determination of PEth in DBS, we developed a fully automated analysis approach. METHODS The validated and novel online-SPE-LC-MS/MS method with automated sample preparation using a CAMAG DBS-MS 500 system reduces manual sample preparation to an absolute minimum, only requiring calibration and quality control DBS. RESULTS During the validation process, the method showed a high extraction efficiency (>88%), linearity (correlation coefficient >0.9953), accuracy and precision (within ±15%) for the determination of PEth 16:0/18:1 and PEth 16:0/18:2. Within a run time of about 7 min, the two monitored analogs could be baseline separated. A method comparison in liquid whole blood of 28 authentic samples from alcohol use disorder patients showed a mean deviation of less than 2% and a correlation coefficient of >0.9759. The comparison with manual DBS extraction showed a mean deviation of less than 8% and a correlation coefficient of >0.9666. CONCLUSIONS The automated analysis of PEth in DBS can provide a fast and accurate solution for abstinence monitoring. In contrast to the manual extraction of PEth in DBS, no laborious sample preparation is required with this automated approach. Furthermore, the application of the internal standard by a spray module can compensate for extraction bias and matrix effects.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute of Legal Medicine
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Management
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Chemistry and Toxicology

UniBE Contributor:

Luginbühl, Marc Joel and Weinmann, Wolfgang

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0146-4760

Publisher:

Oxford University Press

Language:

English

Submitter:

Antoinette Angehrn

Date Deposited:

19 Jul 2019 07:46

Last Modified:

23 Oct 2019 11:53

Publisher DOI:

10.1093/jat/bkz035

PubMed ID:

31062845

BORIS DOI:

10.7892/boris.131246

URI:

https://boris.unibe.ch/id/eprint/131246

Actions (login required)

Edit item Edit item
Provide Feedback