Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process

Contente, Martina Letizia; Farris, Stefano; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2019). Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process. Green Chemistry, 21(12), pp. 3263-3266. Royal Society of Chemistry 10.1039/C9GC01374A

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To increase the uptake of biocatalytic processes by industry, it is essential to demonstrate the reliability of enzyme-based methodologies directly applied to the production of high value products. Here, a unique, efficient, and sustainable enzymatic platform for the multi-gram synthesis of melatonin, projected to generate around 1.5 billion U.S. dollars worldwide by 2021, and its analogues was developed. The system exploits the covalent immobilization of MsAcT (transferase from Mycobacterium smegmatis) onto agarose beads increasing the robustness and longevity of the immobilized biocatalyst. The fully-automated process deriving from the integration between biocatalysis and flow chemistry is designed to maximize the overall yields (58–92%) and reduce reaction times (5 min), overcoming the limitation often associated with bioprocesses and bridging the gap between lab scale and industrial production.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Paradisi, Francesca

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1463-9262

Publisher:

Royal Society of Chemistry

Funders:

[UNSPECIFIED] H2020-MSCA-IF-2017 Project ID 792804

Language:

English

Submitter:

Francesca Paradisi

Date Deposited:

09 Jul 2019 09:55

Last Modified:

23 Oct 2019 18:55

Publisher DOI:

10.1039/C9GC01374A

BORIS DOI:

10.7892/boris.131363

URI:

https://boris.unibe.ch/id/eprint/131363

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