Titanocene binding to oligonucleotides

Eberle, Rahel P.; Schürch, Stefan (2018). Titanocene binding to oligonucleotides. Journal of inorganic biochemistry, 184, pp. 1-7. Elsevier 10.1016/j.jinorgbio.2018.03.014

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The binding of titanocene to DNA and RNA was examined by means of electrospray mass spectrometry. Titanocene served as a model for its therapeutically active derivatives. The binding preferences were probed by competition experiments with oligonucleotides of varying nucleobase compositions and sequences. Results from competition experiments revealed a generally increased preference for the binding to phosphate groups adjacent to thymidines, which is affected by the nucleobase sequence of T-rich oligonucleotides. More detailed information about the binding sites was obtained from tandem mass spectrometric experiments. The binding of the transition metal coordination center significantly altered the fragment ion patterns of the oligonucleotides. RNA was found to be less prone to adduct formation, due to intramolecular interactions. The findings from experiments on DNA and RNA were complemented by the examination of backbone- and ribose-modified oligonucleotides.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Eberle, Rahel and Schürch, Stefan

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0162-0134

Publisher:

Elsevier

Funders:

[42] Schweizerischer Nationalfonds

Language:

English

Submitter:

Stefan Schürch

Date Deposited:

19 Sep 2019 13:22

Last Modified:

05 Nov 2019 10:19

Publisher DOI:

10.1016/j.jinorgbio.2018.03.014

PubMed ID:

29635096

BORIS DOI:

10.7892/boris.131459

URI:

https://boris.unibe.ch/id/eprint/131459

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