Endothelial Progenitor Cells Conditioned Medium Supports Number of GABAergic Neurons and Exerts Neuroprotection in Cultured Striatal Neuronal Progenitor Cells.

Di Santo, Stefano; Seiler, Stefanie; Andres, Robert; Widmer, Hans Rudolf (2019). Endothelial Progenitor Cells Conditioned Medium Supports Number of GABAergic Neurons and Exerts Neuroprotection in Cultured Striatal Neuronal Progenitor Cells. Cell transplantation, 28(4), pp. 367-378. Cognizant Communication Corporation 10.1177/0963689719835192

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There is growing evidence that stem and progenitor cells exert regenerative actions by means of paracrine factors. In line with these notions, we recently demonstrated that endothelial progenitor cell (EPC)-derived conditioned medium (EPC-CM) substantially increased viability of brain microvascular cells. In the present study, we aimed at investigating whether EPC-CM supports cell survival of cultured striatal progenitor cells. For that purpose, primary cultures from fetal rat embryonic (E14) ganglionic eminence were prepared and grown for 7 days (DIV). EPC-CM was administered from DIV5-7. Treatment of the striatal cultures with EPC-CM resulted in significantly increased densities of GABA-immunoreactive (-ir) neurons. Inhibition of mitogen-activated protein kinase and phosphatidylinositol-3-kinase, but not of the ROCK pathway, significantly attenuated the EPC-CM induced increase in GABA-ir cell densities. Similar results were observed when EPC-CM was subjected to proteolytic digestion and lipid extraction. Furthermore, inhibition of translation abolished the EPC-CM induced effects. Importantly, EPC-CM displayed neuroprotection against 3-nitropropionic acid induced toxicity. These findings demonstrate that EPC-derived paracrine factors substantially promote survival and/or differentiation of cultured striatal progenitor cells involving both proteinaceous factors and lipidic factors. In sum, EPC-CM constituents might lead to a novel cell-free therapeutic strategy to challenge neuronal degeneration.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Neurosurgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Mu50 > Forschungsgruppe Neurochirurgie

UniBE Contributor:

Di Santo, Stefano and Widmer, Hans Rudolf

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1555-3892

Publisher:

Cognizant Communication Corporation

Language:

English

Submitter:

Nicole Söll

Date Deposited:

26 Aug 2019 16:22

Last Modified:

24 Oct 2019 03:46

Publisher DOI:

10.1177/0963689719835192

PubMed ID:

31017468

Uncontrolled Keywords:

GABAergic neurons endothelial progenitor cells neuroprotection paracrine factors striatal cultures

BORIS DOI:

10.7892/boris.132405

URI:

https://boris.unibe.ch/id/eprint/132405

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