Petunia- and Arabidopsis-Specific Root Microbiota Responses to Phosphate Supplementation

Bodenhausen, Natacha; Somerville, Vincent; Desirò, Alessandro; Walser, Jean-Claude; Borghi, Lorenzo; van der Heijden, Marcel G. A.; Schlaeppi, Klaus (2019). Petunia- and Arabidopsis-Specific Root Microbiota Responses to Phosphate Supplementation. Phytobiomes journal, 3(2), pp. 112-124. The American Phytopathological Society 10.1094/PBIOMES-12-18-0057-R

[img]
Preview
Text
2019_PhytobiomesJ_3_112.pdf - Published Version
Available under License Creative Commons: Attribution-Noncommercial-No Derivative Works (CC-BY-NC-ND).

Download (2MB) | Preview

Phosphorus (P) is a limiting element for plant growth. Several root microbes, including arbuscular mycorrhizal fungi (AMF), have the capacity to improve plant nutrition and their abundance is known to depend on P fertility. However, how complex root-associated bacterial and fungal communities respond to various levels of P supplementation remains ill-defined. Here we investigated the responses of the root-associated bacteria and fungi to varying levels of P supply using 16S rRNA gene and internal transcribed spacer amplicon sequencing. We grew Petunia, which forms symbiosis with AMF, and the nonmycorrhizal model species Arabidopsis as a control in a soil that is limiting in plant-available P and we then supplemented the plants with complete fertilizer solutions that varied only in their phosphate concentrations. We searched for microbes, whose abundances varied by P fertilization, tested whether a core microbiota responding to the P treatments could be identified and asked whether bacterial and fungal co-occurrence patterns change in response to the varying P levels. Root microbiota composition varied substantially in response to the varying P application. A core microbiota was not identified as different bacterial and fungal groups responded to low-P conditions in Arabidopsis and Petunia. Microbes with P-dependent abundance patterns included Mortierellomycotina in Arabidopsis, while in Petunia, they included AMF and their symbiotic endobacteria. Of note, the P-dependent root colonization by AMF was reliably quantified by sequencing. The fact that the root microbiotas of the two plant species responded differently to low-P conditions suggests that plant species specificity would need to be considered for the eventual development of microbial products that improve plant P nutrition.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Schläppi, Klaus Bernhard

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

2471-2906

Publisher:

The American Phytopathological Society

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 Sep 2019 16:18

Last Modified:

26 Oct 2019 08:07

Publisher DOI:

10.1094/PBIOMES-12-18-0057-R

Uncontrolled Keywords:

Arabidopsis thaliana, arbuscular mycorrhizal fungi, fungal endobacteria, microbiome, Petunia hybrida, phosphate, rhizosphere and phyllosphere, root microbiota

BORIS DOI:

10.7892/boris.132913

URI:

https://boris.unibe.ch/id/eprint/132913

Actions (login required)

Edit item Edit item
Provide Feedback