Stimulation of intervertebral disc cells in alginate bead culture with bone morphogenetic protein 2 and/or L51P

May, Rahel D.; Frauchiger, Daniela A.; Albers, Christoph E.; Benneker, Lorin M.; Hofstetter, Wilhelm; Gantenbein, Benjamin (2019). Stimulation of intervertebral disc cells in alginate bead culture with bone morphogenetic protein 2 and/or L51P. In: 7th International Congress on Biotechnologies for Spinal Surgery (BioSpine7). 3-5 April.

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Introduction: In clinics, Bone Morphogenetic Protein 2 (BMP2) was applied to support spinal fusion. Further BMP2 was tested in IVD models and showed potential for IVD regeneration. The aim of this study is the investigation of BMP2 and L51P on different cell types of the human IVD in 3D alginate beads, particularly their plasticity to undergo bone formation. Methods: Human nucleus pulposus (NPC), annulus fibrosus (AFC) and cartilaginous endplate cells (CEPC) were each encapsulated in 1.2% alginate at a density of 4 Mio/mL. NPC, AFC, and CEPC beads were then cultured in α-MEM or osteogenic medium (OM) supplemented with 10% FBS and 100 ng/mL BMP2 or L51P for seven days. After four days, medium supplemented with cytokines was refreshed. Beads were then snap frozen with liquid nitrogen and analyzed by qPCR and Alcian Blue staining. Results: Aggrecan (ACAN) expression was the highest up-regulated in IVD cells stimulated with OM and 100 ng/ml BMP2 and L51P compared to negative control (basal medium) in NPC, AFC and CEPC (mean ± SEM NP: 18.95 ± 15.65). The same was true for Collagen type 2 (COL2) expression (NP: 72.47 ± 62.95). COL1 remained unaffected (N=2). Conclusion: Recent studies confirmed the anabolic effect of BMP2 on IVD. Also, we showed the trend of an increase in ACAN and COL2 gene expression in stimulated cells. Like in previous studies collagen type 1 (COL1) expression in stimulated IVD cells was unaffected. Interestingly showed the co-treatment of BMP2 and L51P a cumulative effect towards an increased ECM production.

Item Type:

Conference or Workshop Item (Poster)

Division/Institute:

04 Faculty of Medicine > Department of Orthopaedic, Plastic and Hand Surgery (DOPH) > Clinic of Orthopaedic Surgery
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Knochenbiologie & Orthopädische Forschung

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

May, Rahel Deborah; Frauchiger, Daniela Angelika; Albers, Christoph; Benneker, Lorin Michael; Hofstetter, Wilhelm and Gantenbein, Benjamin

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

Language:

English

Submitter:

Benjamin Gantenbein

Date Deposited:

04 Sep 2019 13:34

Last Modified:

23 Oct 2019 08:00

Additional Information:

Proceedings of the 7th International Congress on Biotechnologies for Spinal Surgery (BioSpine7)

BORIS DOI:

10.7892/boris.132964

URI:

https://boris.unibe.ch/id/eprint/132964

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