In vitro characterization of PRRSV isolates with different in vivo virulence using monocyte-derived macrophages.

Ogno, Giulia; Sautter, Carmen Alexandra Nadine; Canelli, Elena; Garcia Nicolas, Obdulio; Stadejek, Tomasz; Martelli, Paolo; Borghetti, Paolo; Summerfield, Artur (2019). In vitro characterization of PRRSV isolates with different in vivo virulence using monocyte-derived macrophages. Veterinary microbiology, 231, pp. 139-146. Elsevier 10.1016/j.vetmic.2019.03.008

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The recent emergence of highly pathogenic porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) strains has caused severe economic losses. The biological elements defining virulence and pathogenicity are still unclear. In vitro characteristics using natural target cells of PRRSV provide important information to understand the basis of virulence at the cellular level, and provide a mean to reduce animal experimentations to achieve this goal. Here, we compared PRRSV strains from two geographically different regions, with varying in vivo characteristics, in terms of their interactions with monocyte-derived macrophages (MDMs). The strains included Lena and BOR59 from Belarus, and ILI6 from Russia, as well as PR11 and PR40, both from Italy. As a reference, we used a cell culture-adapted version of Lelystad, LVP. MDMs were pre-treated with IFNγ, IL-4 or IFNβ, in order to understand responses in polarized and antiviral MDMs. In general, independent of the geographical origin, the strains with high virulence infected a higher percentage of MDMs and replicated to higher titers. These virulence-dependent differences were most pronounced when the MDMs had been treated with IFNβ. Differentiation between intermediate and low virulent PRRSV was difficult, due to variations between different experiments, but LVP differed clearly from all field strains. IFNα and IL-10 were not detected in any experiment, but PR40 induced TNF and IL-1β. Taken together, these results validate the MDM model to understand pathogenicity factors of PRRSV and confirm the importance of the escape from type I and II IFN-mediated effects for PRRSV virulence.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Virology and Immunology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Sautter, Carmen Alexandra Nadine; Garcia Nicolas, Obdulio and Summerfield, Artur

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 630 Agriculture

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Achim Braun Parham

Date Deposited:

04 Sep 2019 17:13

Last Modified:

28 Oct 2019 08:25

Publisher DOI:

10.1016/j.vetmic.2019.03.008

PubMed ID:

30955801

Uncontrolled Keywords:

Interferon Monocyte-derived macrophages PRRSV Virulence

BORIS DOI:

10.7892/boris.132998

URI:

https://boris.unibe.ch/id/eprint/132998

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