Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery

Saurer, Martin; Ramrath, David J. F.; Niemann, Moritz; Calderaro, Salvatore; Prange, Céline; Mattei, Simone; Scaiola, Alain; Leitner, Alexander; Bieri, Philipp; Horn, Elke; Leibundgut, Marc; Boehringer, Daniel; Schneider, André; Ban, Nenad (2019). Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery. Science, 365(6458), pp. 1144-1149. American Association for the Advancement of Science 10.1126/science.aaw5570

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Mitochondrial ribosomes (mitoribosomes) are large ribonucleoprotein complexes that synthesize proteins encoded by the mitochondrial genome. An extensive cellular machinery responsible for ribosome assembly has been described only for eukaryotic cytosolic ribosomes. Here we report that the assembly of the small mitoribosomal subunit in Trypanosoma brucei involves a large number of factors and proceeds through the formation of assembly intermediates, which we analyzed by using cryo-electron microscopy. One of them is a 4-megadalton complex, referred to as the small subunit assemblosome, in which we identified 34 factors that interact with immature ribosomal RNA (rRNA) and recognize its functionally important regions. The assembly proceeds through large-scale conformational changes in rRNA coupled with successive incorporation of mitoribosomal proteins, providing an example for the complexity of the ribosomal assembly process in mitochondria.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry
08 Faculty of Science > Departement of Chemistry and Biochemistry > NCCR RNA & Disease

UniBE Contributor:

Niemann, Moritz; Horn, Elke and Schneider, André

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

0036-8075

Publisher:

American Association for the Advancement of Science

Language:

English

Submitter:

Christina Schüpbach

Date Deposited:

19 Sep 2019 15:25

Last Modified:

03 Nov 2019 23:57

Publisher DOI:

10.1126/science.aaw5570

BORIS DOI:

10.7892/boris.133364

URI:

https://boris.unibe.ch/id/eprint/133364

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